Publication: Production of poly (l-lactide)-degrading enzyme by Actinomadura keratinilytica strain T16-1 under solid state fermentation using agricultural wastes as substrate
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
2190572X
Other identifier(s)
2-s2.0-85119969652
Rights Holder(s)
Scopus
Bibliographic Citation
3 Biotech. Vol 11, No.12 (2021)
Suggested Citation
Krajangsang S., Dechsresawut N., Lomthong T., Samaimai S. Production of poly (l-lactide)-degrading enzyme by Actinomadura keratinilytica strain T16-1 under solid state fermentation using agricultural wastes as substrate. 3 Biotech. Vol 11, No.12 (2021). doi:10.1007/s13205-021-03060-8 Retrieved from: https://hdl.handle.net/20.500.14740/7007
Author(s)
Abstract
Poly (l-lactide) (PLLA) is an aliphatic polyester that can be obtained from renewable resources and degraded by various microorganisms. In previous reports, Actinomadura keratinilytica strain T16-1 demonstrated high ability to degrade PLLA under various conditions. PLLA-degrading enzyme production under solid state fermentation has been sparsely studied. PLLA-degrading enzyme production by A. keratinilytica strain T16-1 was investigated using agricultural wastes as substrate under solid state fermentation (SSF). Three agricultural wastes as soybean meal, cassava chips and duckweed were tested as substrates for PLLA-degrading enzyme production by statistical methods using mixture design. Results revealed that using duckweed as the substrate gave the highest enzyme production (138.66 ± 13.57 U/g dry substrate). Maximum enzyme activity of 391.24 ± 15.57 U/g dry substrate was obtained under 10 g duckweed, 10% inoculum size, 7 days of cultivation time, pH 7.0, 2.8% PLLA powder, and 60% moisture content at 45 °C. It can be concluded that duckweed is an inexpensive substrate, which reduces the costs of PLLA-degrading enzyme production, as an alternative to effective water weed management. © 2021, King Abdulaziz City for Science and Technology.
Subject(s)
Polylactide
Actinomadura
Actinomadura keratinilytica
Agricultural waste
Article
Bacterial strain
Cassava
Controlled study
Enzyme activity
Enzyme degradation
Enzyme synthesis
High temperature
Lemna
Moisture
Nonhuman
Plate count
Powder
Solid state fermentation
Soybean meal
Streptomyces
Streptomyces olivaceus
Submerged fermentation
Surface area
Weed control
Actinomadura
Actinomadura keratinilytica
Agricultural waste
Article
Bacterial strain
Cassava
Controlled study
Enzyme activity
Enzyme degradation
Enzyme synthesis
High temperature
Lemna
Moisture
Nonhuman
Plate count
Powder
Solid state fermentation
Soybean meal
Streptomyces
Streptomyces olivaceus
Submerged fermentation
Surface area
Weed control