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Monoclonal antibodies specific to yellow-head virus (YHV) of Penaeus monodon

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dc.contributor.authorSithigorngul P.
dc.contributor.authorRukpratanporn S.
dc.contributor.authorLongyant S.
dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorSithigorngul W.
dc.contributor.authorMenasveta P.
dc.date.accessioned2021-04-05T04:33:06Z
dc.date.available2021-04-05T04:33:06Z
dc.date.issued2002
dc.date.issuedBE2545
dc.description.abstractMonoclonal antibodies specific to 22, 67 and 135 kDa proteins of yellow-head virus (YHV) were produced from a mouse immunized with partially purified YHV isolated from the haemolymph of experimentally YHV-infected Penaeus monodon. Four groups of monoclonal antibodies were identified. One group of antibodies bound only to native protein of YHV while the others were specific to 135, 67 and 22 kDa proteins in both native and denatured forms. All antibodies could be used to detect YHV infection by means of dot blot and immunohistochemistry. However, antibodies specific to the 22 kDa protein gave the best immunohistochemistry results in terms of intensity and sharpness of staining.
dc.format.mimetypeapplication/pdf
dc.identifier.citationDiseases of Aquatic Organisms. Vol 49, No.1 (2002), p.71-76
dc.identifier.doi10.3354/dao049071
dc.identifier.issn1775103
dc.identifier.other2-s2.0-0037165525
dc.identifier.urihttps://hdl.handle.net/20.500.14740/6735
dc.rights.holderScopus
dc.subject.otherAntibody
dc.subject.otherViral disease
dc.subject.otherMonodon
dc.subject.otherPenaeus monodon
dc.subject.otherPenaeus monodon
dc.subject.otherYellow head virus
dc.subject.otherMonoclonal antibody
dc.subject.otherVirus antibody
dc.subject.otherVirus protein
dc.subject.otherAnimal
dc.subject.otherAnimal disease
dc.subject.otherAntibody specificity
dc.subject.otherArticle
dc.subject.otherEnzyme linked immunosorbent assay
dc.subject.otherHemolymph
dc.subject.otherHybridoma
dc.subject.otherImmunoblotting
dc.subject.otherImmunohistochemistry
dc.subject.otherImmunology
dc.subject.otherIsolation and purification
dc.subject.otherMolecular weight
dc.subject.otherMouse
dc.subject.otherNidovirales
dc.subject.otherPenaeidae
dc.subject.otherVirology
dc.subject.otherAnimals
dc.subject.otherAntibodies, Monoclonal
dc.subject.otherAntibodies, Viral
dc.subject.otherAntibody Specificity
dc.subject.otherEnzyme-Linked Immunosorbent Assay
dc.subject.otherHemolymph
dc.subject.otherHybridomas
dc.subject.otherImmunoblotting
dc.subject.otherImmunohistochemistry
dc.subject.otherMice
dc.subject.otherMolecular Weight
dc.subject.otherNidovirales
dc.subject.otherPenaeidae
dc.subject.otherViral Proteins
dc.titleMonoclonal antibodies specific to yellow-head virus (YHV) of Penaeus monodon
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0037165525&doi=10.3354%2fdao049071&partnerID=40&md5=32137de24af6fab6d826a236550415ea

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