Publication:
Monoclonal antibodies specific to yellow-head virus (YHV) of Penaeus monodon

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Issued Date

2002

Resource Type

Article

File Type

application/pdf

ISSN

1775103

DOI

10.3354/dao049071

Other identifier(s)

2-s2.0-0037165525

Rights Holder(s)

Scopus

Bibliographic Citation

Diseases of Aquatic Organisms. Vol 49, No.1 (2002), p.71-76

Suggested Citation

Sithigorngul P., Rukpratanporn S., Longyant S., Chaivisuthangkura P., Sithigorngul W., Menasveta P. Monoclonal antibodies specific to yellow-head virus (YHV) of Penaeus monodon. Diseases of Aquatic Organisms. Vol 49, No.1 (2002), p.71-76. doi:10.3354/dao049071 Retrieved from: https://hdl.handle.net/20.500.14740/6735

Title

Monoclonal antibodies specific to yellow-head virus (YHV) of Penaeus monodon

Author(s)

Sithigorngul P.
 Rukpratanporn S.
 Longyant S.
 Chaivisuthangkura P.
 Sithigorngul W.
 Menasveta P.

Abstract

Monoclonal antibodies specific to 22, 67 and 135 kDa proteins of yellow-head virus (YHV) were produced from a mouse immunized with partially purified YHV isolated from the haemolymph of experimentally YHV-infected Penaeus monodon. Four groups of monoclonal antibodies were identified. One group of antibodies bound only to native protein of YHV while the others were specific to 135, 67 and 22 kDa proteins in both native and denatured forms. All antibodies could be used to detect YHV infection by means of dot blot and immunohistochemistry. However, antibodies specific to the 22 kDa protein gave the best immunohistochemistry results in terms of intensity and sharpness of staining.

Subject(s)

Antibody
 Viral disease
 Monodon
 Penaeus monodon
 Penaeus monodon
 Yellow head virus
 Monoclonal antibody
 Virus antibody
 Virus protein
 Animal
 Animal disease
 Antibody specificity
 Article
 Enzyme linked immunosorbent assay
 Hemolymph
 Hybridoma
 Immunoblotting
 Immunohistochemistry
 Immunology
 Isolation and purification
 Molecular weight
 Mouse
 Nidovirales
 Penaeidae
 Virology
 Animals
 Antibodies, Monoclonal
 Antibodies, Viral
 Antibody Specificity
 Enzyme-Linked Immunosorbent Assay
 Hemolymph
 Hybridomas
 Immunoblotting
 Immunohistochemistry
 Mice
 Molecular Weight
 Nidovirales
 Penaeidae
 Viral Proteins

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URI

https://hdl.handle.net/20.500.14740/6735

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Scopus: Year 1983-2021

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