Publication: Alternative method for measurement of albumin/creatinine ratio using spectrophotometric sequential injection analysis
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Issued Date
2009
Resource Type
File Type
application/pdf
ISSN
399140
Other identifier(s)
2-s2.0-67650497907
Rights Holder(s)
Scopus
Bibliographic Citation
Talanta. Vol 79, No.4 (2009), p.1111-1117
Suggested Citation
Siangproh W., Teshima N., Sakai T., Katoh S., Chailapakul O. Alternative method for measurement of albumin/creatinine ratio using spectrophotometric sequential injection analysis. Talanta. Vol 79, No.4 (2009), p.1111-1117. doi:10.1016/j.talanta.2008.12.068 Retrieved from: https://hdl.handle.net/20.500.14740/4434
Author(s)
Abstract
A simple, automatic and practical system for successive determination of albumin and creatinine has been developed by combining sequential injection analysis (SIA) and highly sensitive dye-binding assays. Albumin detection was based on the increase in the absorbance due to complex formation between albumin and eosin Y in acidic media. The absorbance of the complex was monitored at 547 nm. For the creatinine assay, the concentration of creatinine was measured by reaction with alkaline picrate to form a colored product which absorbs at 500 nm. The influences of experimental variables such as effects of pH, reagent concentration, standard/sample volume and interferences were investigated. Under optimal conditions, the automated method showed linearity up to 20 mg L-1 for albumin and 100 mg L-1 for creatinine. The 3σ detection limits were 0.6 and 3.5 mg L-1 for albumin and creatinine, respectively, and the relative standard deviations (n = 10) were 2.49% for 20 mg L-1 albumin, and 3.14% for 20 mg L-1 creatinine. Application of the proposed method to the direct analysis of urinary samples yielded results which agreed with those obtained from the Bradford protein assay and a creatinine enzymatic assay according to a paired t-test. The results obtained should be a step towards developing a fully automated and reliable analytical system for clinical research, which requires direct determination of albumin and creatinine and/or its ratios. © 2009 Elsevier B.V. All rights reserved.