Publication:
Nonformalin Fixative Agents: A Comparative Study of Fixative Efficacy and Histomorphology

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

10668969

DOI

10.1177/1066896918779450

Other identifier(s)

2-s2.0-85048089980

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

International Journal of Surgical Pathology. Vol 26, No.8 (2018), p.701-706

Suggested Citation

Lam-Ubol A., Kitrueangphatchara K., Putthanuparp T., Arayakhun R., Kwanthong R., Choonhawarakorn K. Nonformalin Fixative Agents: A Comparative Study of Fixative Efficacy and Histomorphology. International Journal of Surgical Pathology. Vol 26, No.8 (2018), p.701-706. doi:10.1177/1066896918779450 Retrieved from: https://hdl.handle.net/20.500.14740/5761

Title

Nonformalin Fixative Agents: A Comparative Study of Fixative Efficacy and Histomorphology

Author(s)

Lam-Ubol A.
 Kitrueangphatchara K.
 Putthanuparp T.
 Arayakhun R.
 Kwanthong R.
 Choonhawarakorn K.

Abstract

Objectives. Formalin is a gold standard fixative agent. However, formalin possesses health hazards and is not always available in rural areas. The objective of this study was to compare tissue fixative efficacy of nonformalin fixative agents with formalin. Methods. Oral tissues obtained during impacted tooth removal were collected. The tissue fragments were sectioned and fixed in 4 different fixatives; 30% jaggery, 70% ethanol, 2% mepivacaine with 1:100 000 epinephrine, or formalin for 24 and 72 hours. All specimens were then immersed in formalin for another 24 hours and processed according to standard protocol. Hematoxylin and eosin–stained sections were evaluated by a pathologist. The cellular structure, cellular outline, and quality of staining were graded from 1 to 3 and average fixative efficacy scores were compared using 1-way analysis of variance. Fixative artifacts were described. Results. Fixative efficacy scores of 70% ethanol and 30% jaggery at 24 and 72 hours were not statistically different from those of formalin. Conversely, 2% mepivacaine demonstrated significantly lower fixative efficacy scores than other agents. Although efficacy of each fixative was not statistically different between 24 and 72 hours, efficacy of 70% ethanol was markedly reduced at 72 hours when compared with others. Acantholysis of epithelial cells was the most notable artifact at 72 hours when fixed with nonformalin fixative agents. Conclusion. Both 70% ethanol and 30% jaggery provided acceptable fixative efficacy at 24 hours. However, only 30% jaggery maintained fixative efficacy at 72 hours. Nevertheless, negative effects on the epithelial cells were unavoidable and should be interpreted with caution. © The Author(s) 2018.

Subject(s)

Alcohol
 Eosin
 Epinephrine
 Fixative
 Formaldehyde
 Hematoxylin
 Mepivacaine
 Alcohol
 Fixative
 Formaldehyde
 Mepivacaine
 Acantholysis
 Article
 Basement membrane
 Cell junction
 Cell structure
 Comparative study
 Controlled study
 Epithelium cell
 Histomorphology
 Human
 Human cell
 Human tissue
 Inflammatory cell
 Intercellular space
 Mouth tissue
 Pathologist
 Priority journal
 Tissue fixation
 Tooth disease
 Artifact
 Chemistry
 Cytology
 Drug effect
 Mouth mucosa
 Pathology
 Procedures
 Staining
 Tissue fixation
 Tooth disease
 Tooth extraction
 Artifacts
 Epithelial Cells
 Ethanol
 Fixatives
 Formaldehyde
 Humans
 Mepivacaine
 Mouth Mucosa
 Staining and Labeling
 Tissue Fixation
 Tooth Extraction
 Tooth, Impacted

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URI

https://hdl.handle.net/20.500.14740/5761

Collections

Scopus: Year 1983-2021

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