Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/29373
ชื่อเรื่อง: N′-(3-Aminopropyl)-N-(3′-(carbamoyl cholesteryl) propyl)-glycine amide liposomes for delivery of pTRAIL-EGFP
ผู้แต่ง: Weecharangsan W.
Apiratikul N.
Yahuafai J.
Keywords: apoptosis
Cationic liposome
gene delivery
pTRAIL
วันที่เผยแพร่: 2023
สำนักพิมพ์: Taylor and Francis Ltd.
บทคัดย่อ: In this study, N′-(3-aminopropyl)-N-(3′-(carbamoyl cholesteryl) propyl)-glycine amide (A) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE, D) (AD) liposomes were synthesised at molar ratios of 50:25 (AD5025), 50:50 (AD5050) and 50:75 (AD5075) and complexed with plasmid, pTRAIL-EGFP. AD liposome/pTRAIL-EGFP were evaluated for their complex ability, particle size, polydispersity index, zeta potential, expression of pTRAIL-EGFP, cytotoxicity, cell growth inhibition and apoptosis induction in KB cells. AD liposomes complexed completely with pTRAIL-EGFP at AD liposome/DNA ratios of above 4.5/1. The particle size of AD liposome/pTRAIL-EGFP ranged from 180 ± 8 to 1,072 ± 657 nm depending on the proportion of lipid composition and liposome/DNA ratio. The extent of gene expression of pTRAIL-EGFP via AD liposome/pTRAIL-EGFP was significantly higher than that of the cells treated with pTRAIL-EGFP and depended on the AD liposome/DNA ratio. Cytotoxicity of AD liposomes was dependent on A and D molar ratio. Cell growth inhibition of AD liposome/pTRAIL-EGFP was significantly higher than that of the cells treated with pTRAIL-EGFP. The amount of late apoptotic and dead cells of AD liposome/pTRAIL-EGFP was significantly higher than that of cells treated with pTRAIL-EGFP. From this study that one can conclude that AD liposomes can carry and deliver pTRAIL-EGFP into KB cells resulting in cell growth inhibition and cell death. © 2023 Informa UK Limited, trading as Taylor & Francis Group.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85151444401&doi=10.1080%2f08982104.2023.2193638&partnerID=40&md5=d34eb205ca54fc34ec009db0a1691cd0
https://ir.swu.ac.th/jspui/handle/123456789/29373
Appears in Collections:Scopus 2023

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