Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/27414
ชื่อเรื่อง: Antioxidant effect of enamel matrix derivative for early phase of periodontal tissue regeneration in diabetes
ผู้แต่ง: Takeda K.
Mizutani K.
Matsuura T.
Kido D.
Mikami R.
Buranasin P.
Saito N.
Kominato H.
Takemura S.
Nakagawa K.
Iwata T.
Keywords: antioxidant
diabetes mellitus
oxidative stress
periodontal regeneration
wound healing
วันที่เผยแพร่: 2022
สำนักพิมพ์: John Wiley and Sons Inc
บทคัดย่อ: Background: Diabetes involves metabolic disorders in various tissues via hyperglycemia-induced oxidative stress. This study aimed to investigate the antioxidative effect of enamel matrix derivative (EMD) on periodontal regeneration in diabetes. Methods: Twenty-two rats were equally divided into streptozotocin (STZ)-induced diabetes or control group. Two months after induction of hyperglycemia, systemic oxidative stress was measured using urinary 8-hydroxy-2′-deoxyguanosine. EMD or saline was applied into the intrabony defects created in the bilateral maxillary molar. mRNA expressions of inflammatory and oxidative stress markers were quantified (n = 6). Histometric analyses and immunohistochemistry of superoxide dismutase-1 (SOD-1) were performed 7 days postoperatively (n = 5). For in vitro experiments, the bone marrow-derived mesenchymal stem cells were isolated from rat femur and cultured in a high glucose (HG) or control medium. Reactive oxygen species (ROS) measurement and alizarin red staining were performed with/without EMD. Results: Systemic oxidative stress was significantly higher in the diabetic group. The connective tissue attachment and cementum formation were significantly increased at EMD-treated sites in both diabetic and non-diabetic groups. The expression of nicotinamide adenine dinucleotide phosphate oxidase two and four was significantly lower at EMD-treated sites than at EMD-untreated sites in both diabetic and non-diabetic rats. Immunohistochemistry showed significantly higher SOD-1 expression at the EMD-treated site. In vitro, HG culture had significantly higher ROS production compared with control, which was downregulated by EMD. EMD treatment significantly recovered the impaired calcification in HG. Conclusion: EMD promoted early-phase wound healing and periodontal tissue regeneration in the surgically created bony defect of STZ-induced diabetic rat by suppressing hyperglycemia-induced oxidative stress. © 2021 American Academy of Periodontology.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123768556&doi=10.1002%2fJPER.21-0413&partnerID=40&md5=eb82f2d905e24105c0dab44c27b02aec
https://ir.swu.ac.th/jspui/handle/123456789/27414
ISSN: 223492
Appears in Collections:Scopus 2022

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.