Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/27310
ชื่อเรื่อง: Phikud Navakot extract attenuates lipopolysaccharide-induced inflammatory responses through inhibition of ERK1/2 phosphorylation in a coculture system of microglia and neuronal cells
ผู้แต่ง: Temviriyanukul P.
Lertmongkolaksorn T.
Supasawat P.
Pitchakarn P.
Thiyajai P.
Nusuetrong P.
Phochantachinda S.
Chansawhang A.
Chantong B.
Keywords: Gallic acid
Microglia
Mitogen-activated protein kinases pathway
Neuroinflammation
Phikud Navakot
วันที่เผยแพร่: 2022
สำนักพิมพ์: Elsevier Ireland Ltd
บทคัดย่อ: Ethnopharmacological relevance: Phikud Navakot (PN), a mixture of nine herbal plants, is an ancient Thai traditional medicine used for relieving circulatory disorders and dizziness. PN has also shown anti-inflammatory effects in rats with acute myocardial infarction. Moreover, phytochemical-inhibiting neuroinflammation, including gallic acid, vanillic acid, ferulic acid, and rutin were detected in PN extract; however, the anti-neuroinflammatory activity of PN extract and its components in a coculture system of microglia and neuronal cells is limited. Objective: To investigate the anti-neuroinflammatory activities of PN on lipopolysaccharide (LPS)-induced inflammation in a coculture system of microglia and neuronal cells. Methods: ELISA and qRT-PCR were used to assess cytokine expression. The phosphorylation of mitogen-activated protein kinases (MAPKs) was determined by Western blotting. Microglia-mediated neuroinflammation was evaluated using a BV-2 microglia-N2a neuron transwell co-culture. Results: PN extract and its component, gallic acid, decreased LPS-induced the mRNA expression of interleukin-6 (IL-6) and inducible nitric oxide synthase (iNOS), as well as IL-6 protein levels in both microglial monoculture and coculture systems. This was accompanied by a reduction in neurodegeneration triggered by microglia in N2a neurons with increased neuronal integrity markers (βIII tubulin and tyrosine hydroxylase (TH)). These effects were caused by the ability of PN extract to inhibit extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) activation. Conclusion: This is the first study to show that PN extract inhibits neurodegeneration in LPS-activated BV-2 microglia by targeting ERK signaling activity. © 2022 Elsevier B.V.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131448236&doi=10.1016%2fj.jep.2022.115440&partnerID=40&md5=970f5c2ef34e205af556f373ec9e7e87
https://ir.swu.ac.th/jspui/handle/123456789/27310
ISSN: 3788741
Appears in Collections:Scopus 2022

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