Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/17535
ชื่อเรื่อง: Holothuria scabra Extract Induces Cell Apoptosis and Suppresses Warburg Effect by Down-Regulating Akt/mTOR/HIF-1 Axis in MDA-MB-231 Breast Cancer Cells
ผู้แต่ง: Yurasakpong L.
Apisawetakan S.
Pranweerapaiboon K.
Sobhon P.
Chaithirayanon K.
Keywords: actin
alcohol
apoptosis inducing factor
caspase 3
flavanone derivative
glucose
glycolytic enzyme
hexokinase
hexokinase 1
hexokinase 2
hoe 33342
Holothuria scabra extract
hypoxia inducible factor 1alpha
lactic acid
mammalian target of rapamycin
plant extract
protein Bax
protein bcl 2
protein kinase B
triterpenoid
unclassified drug
MTOR protein, human
plant extract
protein kinase B
target of rapamycin kinase
Akt/mTOR signaling
antineoplastic activity
apoptosis
Article
cell viability
chemical composition
chromatin condensation
concentration response
controlled study
down regulation
drug cytotoxicity
drug isolation
drug mechanism
glycolysis
Holothuria
Holothuria scabra
human
human cell
hyperglycemia
IC50
MDA-MB-231 cell line
MTT assay
protein expression
skin fibroblast
triple negative breast cancer
upregulation
Warburg effect
Western blotting
animal
apoptosis
breast tumor
cell proliferation
female
genetics
triple negative breast cancer
tumor cell line
Animals
Apoptosis
Breast Neoplasms
Cell Line, Tumor
Cell Proliferation
Female
Holothuria
Humans
Plant Extracts
Proto-Oncogene Proteins c-akt
TOR Serine-Threonine Kinases
Triple Negative Breast Neoplasms
วันที่เผยแพร่: 2021
บทคัดย่อ: Introduction: Cancer cells utilize the modified glucose metabolism known as Warburg effect, with lactate production as the end product. In the search for alternative therapy, the body wall of sea cucumbers contains various substances with pharmacological activities. Herein, we investigate the effect of Holothuria scabra extract on the viability and Warburg effect of aggressive breast cancer cells. Methods: Body wall of H. scabra was extracted using 95% ethanol. Triple-negative breast cancer cells, MDA-MB-231, were treated with the extract at various concentrations under normoglycemic and hyperglycemic conditions. Cytotoxicity test was performed using MTT assay. Apoptotic proteins were quantified using Western blot. Apoptotic cells were stained with Hoechst 33342. Lactate production was determined using L-lactate assay kit. Results: By MTT assay, H. scabra extract suppressed the viability of breast cancer cells in a dose-dependent and time-dependent manner by enhancing apoptosis, indicated by a marked increase of proapoptotic Bax and pro-caspase three expressions, and decreased expression of anti-apoptotic Bcl-2. The extract could reduce hexokinase II expression, leading to reduced lactate production by blocking the Akt/mTOR/HIF-1 axis. Discussion: Overall findings indicated that H. scabra extract could be a possible therapeutic against breast cancer progression in patients with hyperglycemia, for instance, diabetes mellitus. © 2020 Taylor & Francis Group, LLC.
URI: https://ir.swu.ac.th/jspui/handle/123456789/17535
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85090147639&doi=10.1080%2f01635581.2020.1814825&partnerID=40&md5=2842c7d1f6991014fd17a17da25760cc
ISSN: 1635581
Appears in Collections:Scopus 1983-2021

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