Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/17341
ชื่อเรื่อง: Characterization of stem cells from human ovarian follicular fluid; a potential source of autologous stem cell for cell-based therapy
ผู้แต่ง: Rungsiwiwut R.
Numchaisrika P.
Thuwanut P.
Pruksananonda K.
Keywords: 5' nucleotidase
beta tubulin
CD146 antigen
cytokeratin 18
endoglin
epidermal growth factor
follitropin
Hermes antigen
luteinizing hormone receptor
nestin
o4 protein
octamer transcription factor 4
protein
stage specific embryo antigen 4
stro 1 protein
Thy 1 membrane glycoprotein
transcription factor NANOG
unclassified drug
leukocyte antigen
membrane antigen
nestin
STRO-1 antigen, human
tubulin
adipocyte
Article
autologous stem cell transplantation
cell differentiation
cell isolation
cell structure
chondrocyte
controlled study
female
fibroblast
human
human cell
immunocytochemistry
in vitro study
mesenchymal stem cell
oocyte
osteoblast
ovary follicle fluid
priority journal
protein expression
stem cell culture
biological therapy
cell culture
cell differentiation
cell separation
chemistry
cytology
in vitro fertilization
metabolism
ovary follicle
ovary follicle fluid
physiology
pluripotent stem cell
procedures
regenerative medicine
Antigens, CD
Antigens, Surface
Cell Differentiation
Cell Separation
Cell- and Tissue-Based Therapy
Cells, Cultured
Female
Fertilization in Vitro
Follicular Fluid
Humans
Nestin
Ovarian Follicle
Pluripotent Stem Cells
Regenerative Medicine
Tubulin
วันที่เผยแพร่: 2021
บทคัดย่อ: Human ovarian follicular fluid (HOFF) contains proteins, extracellular matrixes necessary for growth and maturation of oocytes as well as granulosa cells. Epithelial cells and stem cells can be isolated from HOFF. However, information regarding stem cells derived from HOFF is still lacking. The objectives of the present study were to isolate, characterize, and differentiate cells derived from HOFF. HOFF was collected during the routine aspiration of oocytes in an assisted fertilization program and subjected to cell isolation, characterization, and in vitro culture. After 24 h of culture, different cell morphologies including epithelial-like-, neural-like- and fibroblast-like cells were observed. Immunocytochemistry reveals the expression of pluripotent stem cell markers (OCT4, NANOG, SSEA4), epithelial marker (CK18), FSH- and LH-receptor. For in vitro culture, the isolated cells were continuously cultured in a growth medium; alpha MEM containing 10% FBS and epidermal growth factor (EGF). After 2 weeks of in vitro culture, cells with fibroblast-like morphology dominantly grow in the culture vessels and resemble mesenchymal stem cells (MSCs). HOFF-derived cells exhibited MSC expression of CD44, CD73, CD90, CD105, CD146, and STRO-1, and were capable of differentiation into osteoblasts, chondrocytes, and adipocytes. After induction of neural differentiation, HOFF-derived cells formed spheroidal structures and expressed neural stem cell markers including Nestin, β-tubulin III, and O4. Besides, the oocyte-like structure was observed after prolonged culture of HOFF. In conclusion, cells derived from follicular fluid exhibited stem cell characteristics, which could be useful for regenerative medicine applications and cell-based therapies. © 2021, Japan Human Cell Society.
URI: https://ir.swu.ac.th/jspui/handle/123456789/17341
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85100344144&doi=10.1007%2fs13577-020-00439-2&partnerID=40&md5=441b6ef7973571257ca050f4eb9b3389
ISSN: 9147470
Appears in Collections:Scopus 1983-2021

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