Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/17167
ชื่อเรื่อง: Immunogenicity and potential protection of DNA vaccine of Leishmania martiniquensis against Leishmania infection in mice
ผู้แต่ง: Aunguldee T.
Gerdprasert O.
Tangteerawatana P.
Jariyapongskul A.
Leelayoova S.
Wongsatayanon B.T.
Keywords: cell protein
DNA vaccine
gamma interferon
glutamine
glycerol oleate
immunoglobulin G1
immunoglobulin G2a
interleukin 10
penicillin derivative
plasmid DNA
streptomycin
DNA vaccine
IL10 protein, human
interleukin 10
animal experiment
animal model
animal tissue
antibody response
Article
controlled study
cpb gene
Escherichia coli
experimental inflammation
f Leishmania martiniquensis
gene
granuloma
immune response
immunization
immunofluorescence
immunogenicity
immunohistochemistry
inflammation
Leishmania
leishmaniasis
mouse
nonhuman
parasite load
promastigote
protein expression
real time polymerase chain reaction
recombinant plasmid
Th1 cell
animal
Bagg albino mouse
blood
cutaneous leishmaniasis
female
human
immunology
Leishmania
Thailand
vaccination
visceral leishmaniasis
Animals
Female
Humans
Interleukin-10
Leishmania
Leishmaniasis, Cutaneous
Leishmaniasis, Visceral
Mice
Mice, Inbred BALB C
Thailand
Vaccination
Vaccines, DNA
วันที่เผยแพร่: 2021
บทคัดย่อ: Introduction: In Thailand, Leishmania martiniquensis is the predominant species causing cutaneous and visceral leishmaniasis. Its incidence has been increasing among immunocompetent and immunocompromised hosts. We developed a prototype DNA vaccine using a partial consensus sequence of the cysteine protease B (cpb) gene derived from L. martiniquensis from Thai patients. Methodology: The laboratory inbred strain of albino BALB/c mice were immunized intramuscularly three times at 2-week intervals (weeks 0, 2, and 4) with cpb plasmid DNA (pcDNA_cpb) with or without the adjuvant, monoolein (pcDNA_cpb-MO). Mice were challenged at week 6 with L. martiniquensis promastigotes. Sera were analysed for IgG1, IgG2a, interferon gamma and interleukin 10 (IFN-γ and IL-10, respectively) levels at weeks 0, 4, and 9. Additionally, livers and spleens were also analysed for parasite burden using immunohistochemistry and real-time polymerase chain (qPCR) assays. Results: Three weeks after promastigote challenge, vaccinated mice showed significantly increased levels of IgG2a and IFN-γ while IL-10 level was significantly reduced when compared with those in the control group (p < 0.01). Parasite burden in the livers and spleens of vaccinated mice significantly decreased. In addition, a significant increase in mature granuloma formation in the livers when compared with those of the control group (p < 0.05) was found, indicating increased T-helper cells (Th1)-induced inflammation and destruction of amastigotes. Monoolein produced a booster effect to enhance the mouse Th1 protective immunity. Conclusions: The prototype DNA vaccine could induce a Th1 immune response that conferred potential protection to the L. martiniquensis promastigote challenge in BALB/c mice. © 2021 Aunguldee et al.
URI: https://ir.swu.ac.th/jspui/handle/123456789/17167
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85118305440&doi=10.3855%2fjidc.14472&partnerID=40&md5=3bdc637a8c61998d40e60096e2741d82
ISSN: 20366590
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.