Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15320
ชื่อเรื่อง: Lack of specificity of commercially available antisera against muscarinergic and adrenergic receptors
ผู้แต่ง: Pradidarcheep W.
Stallen J.
Labruyère W.T.
Dabhoiwala N.F.
Michel M.C.
Lamers W.H.
Keywords: alpha 2B adrenergic receptor
alpha 2b adrenergic receptor antibody
antiserum
beta 2 adrenergic receptor
beta 2 adrenergic receptor antibody
muscarinic M1 receptor
muscarinic m1 receptor antibody
muscarinic M2 receptor
muscarinic m2 receptor antibody
muscarinic M3 receptor
muscarinic m3 receptor antibody
muscarinic M4 receptor
muscarinic m4 receptor antibody
receptor antibody
unclassified drug
animal cell
antibody specificity
antigen recognition
article
cell membrane
human
human cell
immunohistochemistry
nonhuman
protein expression
rat
Western blotting
Animals
Antibodies
Antibody Specificity
Blotting, Western
Brain Chemistry
Cell Line
Gastrointestinal Tract
Humans
Immune Sera
Immunohistochemistry
Rats
Rats, Wistar
Receptors, Adrenergic
Receptors, Muscarinic
Transfection
Urinary Tract
วันที่เผยแพร่: 2009
บทคัดย่อ: Commercially available antisera against five subtypes of muscarinic receptors and nine subtypes of adrenoceptors showed highly distinct immunohistochemical staining patterns in rat ureter and stomach. However, using the M1-4 muscarinic receptor subtypes and α2B-, β2-, and β3-adrenoceptors as examples, Western blots with membranes prepared from cell lines stably expressing various subtypes of muscarinic receptors or adrenoceptors revealed that each of the antisera recognized a set of proteins that differed between the cell lines used but lacked specificity for the claimed target receptor. We propose that receptor antibodies need better validation before they can reliably be used.
URI: https://ir.swu.ac.th/jspui/handle/123456789/15320
https://www.scopus.com/inward/record.uri?eid=2-s2.0-60849088554&doi=10.1007%2fs00210-009-0393-0&partnerID=40&md5=d0e8080079d633313ad4db818bf0a295
ISSN: 281298
Appears in Collections:Scopus 1983-2021

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