Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/15293
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dc.contributor.authorThanomsub B.W.
dc.contributor.authorChansiri K.
dc.contributor.authorSarataphan N.
dc.contributor.authorPhantana S.
dc.date.accessioned2021-04-05T04:33:26Z-
dc.date.available2021-04-05T04:33:26Z-
dc.date.issued2000
dc.identifier.issn8908508
dc.identifier.other2-s2.0-0034067693
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0034067693&doi=10.1006%2fmcpr.1999.0283&partnerID=40&md5=e38398060df019e0cd5e2923b2c64fea
dc.identifier.urihttp://ir.swu.ac.th/jspui/handle/123456789/15293-
dc.description.abstractFilariasis is still a public health problem in tropical countries. The most common causative agents of human filariasis are Wuchereria bancrofti and Brugia malayi. Traditional methods used to detect filarial parasites in human, animal and vector populations are tedious, time consuming, and confer little guarantee of sensitivity and species specificity. We have developed a rapid and specific method to detect filarial parasite DNAs in blood and mosquito samples using the polymerase chain reaction (PCR) technique. The primers used are MF/F and MF/R which amplify a 1.5 kb glutathione peroxidase gene of filarial worms. Using the restriction fragment length polymorphism (RFLP) technique, these PCR products will be further digested with restriction enzymes either HpaI, PstI, AluI or HinfI to differentiate the genus of filaria. This PCR-RFLP technique can be apply to use in diagnosis and to differentiate between species of filaria in humans the reservoir host and the mosquito vector in endemic areas. (C) 2000 Academic Press.
dc.subjectethidium bromide
dc.subjectglutathione peroxidase
dc.subjectrestriction endonuclease
dc.subjectarticle
dc.subjectblood analysis
dc.subjectBrugia malayi
dc.subjectcat
dc.subjectcontrolled study
dc.subjectdifferential diagnosis
dc.subjectDNA determination
dc.subjectfilariasis
dc.subjectgene amplification
dc.subjecthuman
dc.subjectmosquito
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectrestriction fragment length polymorphism
dc.subjectWuchereria bancrofti
dc.subjectAnimalia
dc.subjectBrugia malayi
dc.subjectFelis catus
dc.subjectFilaria
dc.subjectNematoda
dc.subjectWuchereria bancrofti
dc.titleDifferential diagnosis of human lymphatic filariasis using PCR-RFLP
dc.typeArticle
dc.rights.holderScopus
dc.identifier.bibliograpycitationMolecular and Cellular Probes. Vol 14, No.1 (2000), p.41-46
dc.identifier.doi10.1006/mcpr.1999.0283
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