Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15290
ชื่อเรื่อง: Hyposerotonin-induced nitric oxide supersensitivity in the cerebral microcirculation
ผู้แต่ง: Srikiatkhachorn A.
Anuntasethakul T.
Maneesri S.
Phansuwan-Pujito P.
Patumraj S.
Kasantikul V.
Keywords: nitric oxide
animal cell
animal experiment
artery dilatation
article
blood vessel reactivity
brain circulation
controlled study
male
migraine
nonhuman
pia artery
priority journal
rat
serotoninergic system
supersensitivity
vasodilatation
vasomotor reflex
Animals
Arterioles
Brain
Cerebrovascular Circulation
Disease Models, Animal
Male
Migraine Disorders
Nitric Oxide
Rats
Rats, Inbred WF
Serotonin
วันที่เผยแพร่: 2000
บทคัดย่อ: Objective.-To investigate the relationship between hyposerotonin and cranial microvascular responses to nitric oxide (NO). Background.-Although the mechanism underlying NO supersensitivity in migraine is still unclear, an alteration of the serotonin system is a possible explanation. Methods.-Wistar rats were divided into control and hyposerotonin groups. Serotonin was depleted by intraperitoneal injection with 300 mg/kg of para- chlorophenylalanine (PCPA), a tryptophan hydroxylase inhibitor. Three days after PCPA pretreatment, the animals were prepared for assessment of their NO-induced vasomotor response using glyceryl trinitrate (GTN: 8 to 10 mg/kg, intravenously) as an NO donor. Pial circulation was visualized by the intravital fluorescein videomicroscopic technique. Images of vessels at 0, 5, 15, 30, and 60 minutes post GTN infusion were digitized and measured. At the end of monitoring, the rat brains were removed for ultrastructural study of the brain microvessels. Results.-Infusion of GTN produced dose-dependent piaI arteriolar dilatation. This vasodilator effect was significantly increased in the PCPA-treated groups, especially at 30 and 60 minutes. The percentage change from baseline diameter at 30 minutes after the 8-mg/kg GTN infusion was 42.6 ± 3.1 for the hyposerotonin group and 16.8 ± 2.9 for the control group (P<.001). Electron microscopic study revealed that exposure to the NO donor produced considerable changes in cerebral microvessels, characterized by focal ballooning of endothelial cells, increased microvillous formation, and increased endothelial pinocytosis. These anatomical changes were significantly more prominent in the hyposerotonin group. Conclusions.-A hyposerotoninergic condition can facilitate the NO-induced physiological and pathological responses in meningeal and cerebral microvessels and, therefore, is a possible explanation for the supersensitivity to NO observed in patients with migraine.
URI: https://ir.swu.ac.th/jspui/handle/123456789/15290
https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033993718&doi=10.1046%2fj.1526-4610.2000.00040.x&partnerID=40&md5=d5db178bdab65fe317855fdfc76a8354
ISSN: 178748
Appears in Collections:Scopus 1983-2021

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