Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15276
ชื่อเรื่อง: Melatonin reduces induction of Bax, caspase and cell death in methamphetamine-treated human neuroblastoma SH-SY5Y cultured cells
ผู้แต่ง: Wisessmith W.
Phansuwan-Pujito P.
Govitrapong P.
Chetsawang B.
Keywords: caspase 3
desipramine
melatonin
methamphetamine
protein Bax
protein bcl 2
article
cell protection
cell viability
controlled study
enzyme degradation
human
human cell
mitochondrion
nerve cell degeneration
nerve cell necrosis
neuroblastoma cell
neuroprotection
bcl-2-Associated X Protein
Caspase 3
Cell Count
Cell Death
Cell Line, Tumor
Cell Survival
Desipramine
Drug Interactions
Humans
Melatonin
Methamphetamine
Microscopy, Fluorescence
Mitochondria
Neuroblastoma
Neuroprotective Agents
Signal Transduction
วันที่เผยแพร่: 2009
บทคัดย่อ: Several studies demonstrated that methamphetamine (MA)-treated human neuroblastoma cells exhibit increased oxidative stress, which regulates intracellular signaling cascades leading to cell death. Melatonin has a potential as a direct free radical scavenger and protects against cell death caused by MA. The objective of this study was to investigate the neuroprotective properties of melatonin on MA-induced induction of death signaling cascade and neuronal cell degeneration in human neuroblastoma SH-SY5Y cultured cells. The results of the present study demonstrate that MA significantly reduced cell viability in SH-SY5Y cultured cells. Desipramine, a monoamine uptake blocker, and melatonin reversed the toxic effect of MA in reducing cell viability. Induction of Bax, Bcl-2 and cleaved caspase-3 protein levels were observed in SH-SY5Y cultured cells treated with MA, whereas the induction of Bax and cleaved caspase-3 was diminished by melatonin. Visualization of the induction of Bax using immunofluorescence but a reduction in mitochondrial sites using red-fluorescent mitochondria-staining dye was more obviously apparent in MA-treated cells than in untreated control cells and, again, this effect was abolished by melatonin. These findings demonstrate important roles of Bax and caspase in death signaling cascade, and the protective effects of melatonin in MA-treated SH-SY5Y cells. © 2009 Blackwell Munksgaard.
URI: https://ir.swu.ac.th/jspui/handle/123456789/15276
https://www.scopus.com/inward/record.uri?eid=2-s2.0-64649097900&doi=10.1111%2fj.1600-079X.2009.00680.x&partnerID=40&md5=57ed2cf999800e2d2083ca22bf6f2222
ISSN: 7423098
Appears in Collections:Scopus 1983-2021

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