Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15212
ชื่อเรื่อง: Four novel PYFs: Members of NPY/PP peptide superfamily from the eyestalk of the giant tiger prawn Penaeus monodon
ผู้แต่ง: Sithigorngul P.
Pupuem J.
Krungkasem C.
Longyant S.
Panchan N.
Chaivisuthangkura P.
Sithigorngul W.
Petsom A.
Keywords: acetic acid
amide
arginylalanylarginylprolylarginylphenylalaninamide
hexapeptide
methanol
monoclonal antibody
neuropeptide
neuropeptide Y
nonapeptide
pancreas polypeptide
phenylalanylmethionylarginylphenylalaninamide like peptide
tyrosylalanylisoleucylalanylglycylarginylprolylarginylphenylalaninamide
tyrosylphenylalanine
tyrosylserylglutaminylvalylserylarginylprolylarginylphenylalaninamide
tyrosylserylleucylarginylalanylarginylprolylarginylphenylalaninamide
unclassified drug
water
neuropeptide Y
amino acid sequence
animal tissue
article
brain region
carboxy terminal sequence
controlled study
enzyme linked immunosorbent assay
ganglion
high performance liquid chromatography
immunocompetent cell
immunocytochemistry
invertebrate
nonhuman
perikaryon
priority journal
protein analysis
protein family
protein isolation
protein localization
protein purification
protein structure
sequence analysis
animal
chemistry
eye
immunohistochemistry
isolation and purification
molecular genetics
Penaeidae
sequence homology
Cephalopoda
Invertebrata
Loligo
Loligo vulgaris
Monodon
Panthera tigris
Penaeus monodon
Vertebrata
Amino Acid Sequence
Animals
Enzyme-Linked Immunosorbent Assay
Eye
Immunohistochemistry
Molecular Sequence Data
Neuropeptide Y
Penaeidae
Sequence Homology, Amino Acid
วันที่เผยแพร่: 2002
บทคัดย่อ: An immunocytochemical method was used for localization of pancreatic polypeptide (PP) immunoreactive substances in the eyestalk of Penaeus monodon using anti-C-terminal hexapeptide of PP (anti-PP6) antiserum. Approximately 200 neuronal cell bodies were recognized in the ganglia between the medulla interna (MI) and medulla terminalis (MT) and surrounding MT in conjunction with the neuronal processes in medulla externa (ME), MI, MT and sinus gland. About half of the PP immunoreactive neurons were also recognized by a combination of three monoclonal antibodies raised against FMRFamide-like peptides. Isolation of the PP immunoreactive substances from the eyestalk was performed using 7500 eyestalks extracted in methanol/acetic acid/water (90/1/9) followed by five to six steps of RP-HPLC separation. Dot-ELISA with anti-PP6 antiserum was used to monitor PP-like substances in various fractions during the purification processes. Four new sequences of one hexapeptide; RARPRFamide, and three nonapeptides; YSQVSRPRFamide, YAIAGRPRFamide and YSLRARPRFamide were identified, and named as Pem-PYF1-4 due to their structural similarity to the PYF found in squid Loligo vulgaris. Each of the new peptides shares four to seven common residues with the C-terminus of the squid PYF and with the NPFs found in other invertebrates. The NPY/PP superfamily as well as the FMRFamide peptide family may be present throughout vertebrates and invertebrates. © 2002 Elsevier Science Inc. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/15212
https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036849337&doi=10.1016%2fS0196-9781%2802%2900176-6&partnerID=40&md5=0bc6a2f78dac6a347f7c8af645cfd68a
ISSN: 1969781
Appears in Collections:Scopus 1983-2021

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