Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15102
ชื่อเรื่อง: Involvement of reactive oxygen species and stress-activated MAPKs in satratoxin H-induced apoptosis
ผู้แต่ง: Nusuetrong P.
Yoshida M.
Tanitsu M.-A.
Kikuchi H.
Mizugaki M.
Shimazu K.-I.
Pengsuparp T.
Meksuriyen D.
Oshima Y.
Nakahata N.
Keywords: 2 (2 amino 3 methoxyphenyl)chromone
4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole
acetylcysteine
anthra[1,9 cd]pyrazol 6(2h) one
glutathione
glutathione reductase
mitogen activated protein kinase
mitogen activated protein kinase p38
mycotoxin
reactive oxygen metabolite
satratoxin h
stress activated protein kinase
unclassified drug
animal cell
apoptosis
article
cell stimulation
cell strain
chromatin structure
coculture
controlled study
cytopathogenic effect
drug inhibition
enzyme activation
flow cytometry
nonhuman
priority journal
protein phosphorylation
rat
staining
Animals
Apoptosis
Cell Survival
Dose-Response Relationship, Drug
Mitogen-Activated Protein Kinases
Mycotoxins
PC12 Cells
Rats
Reactive Oxygen Species
Stress
Trichothecenes
วันที่เผยแพร่: 2005
บทคัดย่อ: Satratoxins, members of the trichothecene mycotoxin family, have been known to be harmful to health. However, the mechanisms underlying the toxicity still remain unclear. The present study is undertaken to elucidate the mechanisms of the satratoxin H-induced cytotoxicity in PC12 cells. Satratoxin H caused cytotoxicity, which was reflected from apoptosis determined by chromatin staining and flow cytometry. Satratoxin H stimulated the phosphorylation of extracellular signal-regulated kinase (ERK), p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK). Pre-incubation with SB203580, a p38 MAPK inhibitor, or SP600125, a JNK inhibitor, but not PD98059, an ERK inhibitor, reduced satratoxin-induced cytotoxicity. Co-incubation of cells with glutathione, N-acetyl-l-cysteine or glutathione reductase inhibited cytotoxicity and the phosphorylation of p38 MAPK induced by satratoxin H. Our data suggest that satratoxin H-induced apoptosis in PC12 cells is dependent on the activation of p38 MAPK/JNK and the increase in reactive oxygen species. © 2004 Elsevier B.V. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/15102
https://www.scopus.com/inward/record.uri?eid=2-s2.0-19944431322&doi=10.1016%2fj.ejphar.2004.11.046&partnerID=40&md5=924da287a512dfb05b8ef5463585e248
ISSN: 142999
Appears in Collections:Scopus 1983-2021

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