Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/15099
ชื่อเรื่อง: Production of monoclonal antibodies for detection of Vibrio harveyi
ผู้แต่ง: Phianphak W.
Rengpipat S.
Rukpratanporn S.
Longyant S.
Chaivisuthangkura P.
Sithigorngul W.
Sithigorngul P.
Keywords: antibody
วันที่เผยแพร่: 2005
บทคัดย่อ: Monoclonal antibodies (MAbs) against Vibrio harveyi were produced from mice immunized with heat-killed and SDS-mercaptoethanol-treated highly virulent V. harveyi 639. Fifteen MAbs were selected and sorted into 6 groups according to their specificity to various proteins of apparent molecular weight ranging from 8 to 49 kDa. Some antibodies were used for detection of V. harveyi at concentrations as low as 104 CFU ml-1 using immunodot blots. Most of the selected MAbs did not show cross-reactivity to other Vibrio species and other Gram-negative bacteria tested. Only 1 MAb (VH39-4E) showed slight cross-reactivity to Aeromonas hydrophila. Another MAb (VH24-8H) bound lightly to V. harveyi 1526 but strongly to V. harveyi 639, allowing rapid differentiation. Two of the MAb groups were used to localize V. harveyi in tissues of infected black tiger shrimp Penaeus monodon by immunohistochemistry. This study demonstrates the versatility of a highly specific immunological tool for the detection of V. harveyi in aquaculture and opens the way for further development of convenient test kits. © Inter-Research 2005.
URI: https://ir.swu.ac.th/jspui/handle/123456789/15099
https://www.scopus.com/inward/record.uri?eid=2-s2.0-16344364046&doi=10.3354%2fdao063161&partnerID=40&md5=4c25c8c978c731442acb87aa081aefae
ISSN: 1775103
Appears in Collections:Scopus 1983-2021

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