Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14788
ชื่อเรื่อง: IL4 gene polymorphism and previous malaria experiences manipulate anti-Plasmodium falciparum antibody isotype profiles in complicated and uncomplicated malaria
ผู้แต่ง: Tangteerawatana P.
Perlmann H.
Hayano M.
Kalambaheti T.
Troye-Blomberg M.
Khusmith S.
Keywords: immunoglobulin E antibody
immunoglobulin G antibody
interleukin 4
immunoglobulin class
immunoglobulin E
immunoglobulin G
interleukin 4
adolescent
adult
aged
article
controlled study
disease severity
disease transmission
endemic disease
enzyme linked immunosorbent assay
female
genetic variability
genotype
human
major clinical study
malaria
male
Plasmodium falciparum
restriction fragment length polymorphism
single nucleotide polymorphism
antibody production
blood
genetic variability
genetics
hospitalization
immunology
malaria falciparum
middle aged
parasitology
polymerase chain reaction
Adolescent
Adult
Aged
Antibody Formation
Enzyme-Linked Immunosorbent Assay
Female
Genetic Variation
Genotype
Humans
Immunoglobulin E
Immunoglobulin G
Immunoglobulin Isotypes
Interleukin-4
Malaria, Falciparum
Male
Middle Aged
Plasmodium falciparum
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Severity of Illness Index
Young Adult
วันที่เผยแพร่: 2009
บทคัดย่อ: Abstract. Background. The IL4-590 gene polymorphism has been shown to be associated with elevated levels of anti-Plasmodium falciparum IgG antibodies and parasite intensity in the malaria protected Fulani of West Africa. This study aimed to investigate the possible impact of IL4-590C/T polymorphism on anti-P. falciparum IgG subclasses and IgE antibodies levels and the alteration of malaria severity in complicated and uncomplicated malaria patients with or without previous malaria experiences. Methods. Anti-P.falciparum IgG subclasses and IgE antibodies in plasma of complicated and uncomplicated malaria patients with or without previous malaria experiences were analysed using ELISA. IL4-590 polymorphisms were genotyped using RFLP-PCR. Statistical analyses of the IgG subclass levels were done by Oneway ANOVA. Genotype differences were tested by Chi-squared test. Results. The IL4-590T allele was significantly associated with anti-P. falciparum IgG3 antibody levels in patients with complicated (P = 0.031), but not with uncomplicated malaria (P = 0.622). Complicated malaria patients with previous malaria experiences carrying IL4-590TT genotype had significantly lower levels of anti-P. falciparum IgG3 (P = 0.0156), while uncomplicated malaria patients with previous malaria experiences carrying the same genotype had significantly higher levels (P = 0.0206) compared to their IL4-590 counterparts. The different anti-P. falciparum IgG1 and IgG3 levels among IL4 genotypes were observed. Complicated malaria patients with previous malaria experiences tended to have lower IgG3 levels in individuals carrying TT when compared to CT genotypes (P = 0.075). In contrast, complicated malaria patients without previous malaria experiences carrying CC genotype had significantly higher anti-P. falciparum IgG1 than those carrying either CT or TT genotypes (P = 0.004, P = 0.002, respectively). Conclusion. The results suggest that IL4-590C or T alleles participated differently in the regulation of anti-malarial antibody isotype profiles in primary and secondary malaria infection and, therefore, could play an important role in alteration of malaria severity. © 2009 Tangteerawatana et al; licensee BioMed Central Ltd.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14788
https://www.scopus.com/inward/record.uri?eid=2-s2.0-74549211889&doi=10.1186%2f1475-2875-8-286&partnerID=40&md5=836747fa18b1a6c0624b217eb7b4f82e
ISSN: 14752875
Appears in Collections:Scopus 1983-2021

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