Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14719
ชื่อเรื่อง: Cloning and expression of 1-deoxy-d-xylulose 5-phosphate synthase cDNA from Croton stellatopilosus and expression of 2C-methyl-d-erythritol 4-phosphate synthase and geranylgeranyl diphosphate synthase, key enzymes of plaunotol biosynthesis
ผู้แต่ง: Sitthithaworn W.
Wungsintaweekul J.
Sirisuntipong T.
Charoonratana T.
Ebizuka Y.
De-Eknamkul W.
Keywords: 1 deoxy D xylulose 5 phosphate reductoisomerase
1-deoxy-D-xylulose 5-phosphate reductoisomerase
complementary DNA
deoxyxylulose 5 phosphate synthase
deoxyxylulose-5-phosphate synthase
farnesyl trans transferase
fatty alcohol
isomerase
messenger RNA
multienzyme complex
oxidoreductase
plaunotol
transferase
amino acid sequence
antibody specificity
article
chemistry
Croton
enzymology
gene expression profiling
gene expression regulation
genetics
metabolism
molecular cloning
molecular genetics
nucleotide sequence
phylogeny
plant
plant leaf
sequence alignment
ultrastructure
Aldose-Ketose Isomerases
Amino Acid Sequence
Cloning, Molecular
Croton
DNA, Complementary
Farnesyltranstransferase
Fatty Alcohols
Gene Expression Profiling
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Molecular Sequence Data
Multienzyme Complexes
Organ Specificity
Oxidoreductases
Phylogeny
Plant Leaves
Plant Shoots
RNA, Messenger
Sequence Alignment
Transferases
Arabidopsis
Arabidopsis thaliana
Embryophyta
Escherichia coli
วันที่เผยแพร่: 2010
บทคัดย่อ: 1-Deoxy-d-xylulose 5-phosphate synthase (DXS, EC: 4.1.3.37), the first enzyme in the 2C-methyl-d-erythritol 4-phosphate (MEP) pathway, is known to be responsible for the rate-limiting step of isoprenoid biosynthesis in Escherichia coli and Arabidopsis thaliana. In this study, the dxs gene from Croton stellatopilosus, designated csdxs, was cloned from leaf tissue using the rapid amplification of cDNA ends (RACE) technique. Leaves of C. stellatopilosus contain plaunotol, an acyclic diterpene alcohol. The csdxs cDNA containing the open reading frame of 2163 base pairs appeared to encode a polypeptide of 720 amino acids. Analysis of the deduced amino acid sequence revealed that the NH2-terminus of CSDXS carried a chloroplast transit peptide, a thiamine diphosphate binding site, and a transketolase motif, which are the important characteristics of DXS enzymes in higher plants. Multiple alignments of CSDXS with other plant DXSs have indicated that CSDXS has identity ranging between 68% and 89%. Expression levels of csdxs and genes encoding key enzymes in the plaunotol biosynthetic pathway, namely 2C-methyl-d-erythritol 4-phosphate synthase (meps) and geranylgeranyl diphosphate synthase (ggpps), were analysed by measuring transcript levels in leaves of different developmental stages. The results showed that dxs, meps, and ggpps are all active in young leaves prior to full expansion when plaunotol is synthesised from the DXP precursor in chloroplasts. The dense presence of chloroplasts and oil globules in the palisade cells of these leaves support the view that these genes are involved in plaunotol biosynthesis in chloroplast-containing tissues. Crown Copyright © 2009.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14719
https://www.scopus.com/inward/record.uri?eid=2-s2.0-75149166956&doi=10.1016%2fj.jplph.2009.09.001&partnerID=40&md5=58a66439b440826c4021bd6082ed1c6c
ISSN: 1761617
Appears in Collections:Scopus 1983-2021

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