Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14633
ชื่อเรื่อง: Influence of capsicum extract and capsaicin on endothelial health
ผู้แต่ง: Chularojmontri L.
Suwatronnakorn M.
Wattanapitayakul S.K.
Keywords: capsaicin
endothelial nitric oxide synthase
lipopolysaccharide
nitric oxide
NOS3 protein, human
plant extract
apoptosis
article
biosynthesis
cell culture
chemistry
cytology
dose response
drug effect
endothelium cell
health
human
male
metabolism
newborn
oxidative stress
pepper
umbilical cord
umbilical vein
Western blotting
Apoptosis
Blotting, Western
Capsaicin
Capsicum
Cells, Cultured
Dose-Response Relationship, Drug
Endothelial Cells
Health
Humans
Infant, Newborn
Lipopolysaccharides
Male
Nitric Oxide
Nitric Oxide Synthase Type III
Oxidative Stress
Plant Extracts
Umbilical Cord
Umbilical Veins
วันที่เผยแพร่: 2010
บทคัดย่อ: Objective: To examine the effect of Capsicum spp extract (CEX) and capsaicin (CAP) on endothelial nitric oxide release and protection against lipopolysaccharide (LPS)-induced cellular apoptosis. Material and Method: Human umbilical vein endothelial cells (HUVEC) were isolated from newborn cords. Evaluation of cytotoxicity was performed by MTT assay. Endothelial nitric oxide (NO) production was evaluated by Griess reaction. Alteration in eNOS expression was detected by westernblot analysis. To induce oxidative stress and apoptosis, lipopolysaccharide (LPS) was coincubated with HUVEC in the presence or absence of CEX or CAP, and the vanilloid receptor blocker capsazepine (CZP). Hoechst nuclear staining was used to determine percent apoptotic nuclei. Results: The highest concentrations of CEX (1000 μg/mL) and CAP (25 μM) used in the study did not induce cytotoxicity in HUVEC. Significant increase in NO release was observed when cells were incubated with CEX (100 μg/mL) and CAP (25 μM) and this effect was inhibited by CZP only in CAP treatment group. Despite enhanced NO generation was observed, western blot analysis indicated no change in eNOS expression. Interestingly, endothelial cells incubated with L-arginine (LARG, 1000 μg/mL) alone significantly showed increased NO production while L-ARG co-incubation abrogated CEX or CAP effects on endothelial NO generation. CEX (10 μg/mL) and CAP (1μM) decreased apoptotic nuclei in HUVEC treated with LPS. Conclusion: CEX and CAP improved endothelial function and protected against LPS-induced apoptosis. Regular consumption of Capsicum spp. may promote endothelial health and reduce cardiovascular disease risk.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14633
https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952271566&partnerID=40&md5=f93103f2f565d512f1e6373792d98d34
ISSN: 1252208
Appears in Collections:Scopus 1983-2021

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