Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14619
Title: Anticancer and apoptosis-inducing activities of microbial metabolites
Authors: Phonnok S.
Tanechpongtamb W.U.
Wongsatayanon B.T.
Keywords: Anti-cancer agents
Anti-proliferative
Anticancer
Anticancer activities
Apoptosis-inducing activity
Bioactive compounds
Cancer cell lines
Cancer cells
Cancer Chemotherapy
Caspase-3
Caspases
Cell extracts
Cell-shrinkage
DNA cleavage
DNA ladder
DNA-binding
Drug resistance
Gel electrophoresis
Growth inhibition
High concentration
Microbial products
Morphological changes
MTT assays
Surface contact
Cell culture
Chemotherapy
Diseases
DNA
Electrophoresis
Lead compounds
Metabolism
Metabolites
Cell death
caspase 3
DNA
doxorubicin
microbial products not classified elsewhere
Acinetobacter baumannii
animal cell
antineoplastic activity
apoptosis
article
Bacillus
cancer cell culture
cancer inhibition
Candida tropicalis
cell proliferation
cell structure
concentration response
controlled study
DNA cleavage
DNA fragmentation
drug cytotoxicity
drug isolation
drug potency
drug screening
drug specificity
enzyme activation
IC 50
nonhuman
Pseudomonas aeruginosa
Issue Date: 2010
Abstract: The problems of systemic toxicity and drug resistance in cancer chemotherapy urge the continuing discovery of new anticancer agents. We explored the specific anticancer activity from microbial metabolites to find new lead compound. 394 microbial extracts were evaluated on anti-proliferative activity against 4 cancer cell lines using MTT assay. Of these, 20 samples showed varying degree of cytotoxicity but specifically to the cancer cell lines since the growth of normal cells was not significantly inhibited by 1 mg/ml of each cell extracts. The 4 most potent extracts exhibited strongest growth inhibition to each cancer cell type were selected for further studied. Cell morphological changes such as cell shrinkage, lose of surface contact and blebbing were observed in all treated cancer cells. DNA-binding dye staining demonstrated nuclear condensation and fragmentation. Chromosomal DNA cleavage detected as DNA ladder pattern by gel electrophoresis including activation of cellular caspase-3 activity, a hallmark of apoptosis, were observed in all treated cancer cell lines. These characteristics suggested the mechanism of apoptosis cell death induced by the extracts. No growth inhibition and apoptosis characteristic were detected in normal cells even at high concentration used suggesting the selective cytotoxicity and potential candidates to develop as anticancer agents. © 2010 by Pontificia Universidad Católica de Valparaíso, Chile.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14619
https://www.scopus.com/inward/record.uri?eid=2-s2.0-79960762004&doi=10.2225%2fvol13-issue5-fulltext-7&partnerID=40&md5=389d8d98120c55724b28631a2074341a
ISSN: 7173458
Appears in Collections:Scopus 1983-2021

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