Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14547
ชื่อเรื่อง: HeLa cell transfection using a novel sonoporation system
ผู้แต่ง: Rodamporn S.
Harris N.R.
Beeby S.P.
Boltryk R.J.
Sanchez-Elsner T.
Keywords: At resonance
Cell transfection
Cell viability
Chamber design
HeLa cell
High rate
Material thickness
PZT
Resonant frequencies
sonoporation
ultrasonic standing wave
Ultrasonic standing waves
Drug delivery
Elastic waves
Gene therapy
Genetic engineering
Natural frequencies
Regression analysis
Transducers
Ultrasonic waves
Waves
Ultrasonics
plasmid DNA
article
cell viability
controlled study
female
genetic transfection
HeLa cell
human
human cell
human cell culture
ionization chamber
piezoelectric transducer
piezoelectricity
plasmid
sonoporation
transducer
ultrasound
Electroporation
Equipment Design
Equipment Failure Analysis
Hela Cells
Humans
Microfluidic Analytical Techniques
Sonication
Transfection
วันที่เผยแพร่: 2011
บทคัดย่อ: Sonoporation has been shown to have an important role in biotechnology for gene therapy and drug delivery. This paper presents a novel microfluidic sonoporation system that achieves high rates of cell transfection and cell viability by operating the sonoporation chamber at resonance. The paper presents a theoretical analysis of the resonant sonoporation chamber design, which achieves sonoporation by forming an ultrasonic standing wave across the chamber. A piezoelectric transducer (PZT 26) is used to generate the ultrasound and the different material thicknesses have been identified to give a chamber resonance at 980 kHz. The efficiency of the sonoporation system was determined experimentally under a range of sonoporation conditions and different exposures time (5, 10, 15, and 20 s, respectively) using HeLa cells and plasmid (peGFP-N1). The experimental results achieve a cell transfection efficiency of 68.9% (analysis of variance, ANOVA, p lt; 0.05) at the resonant frequency of 980 kHz at 100 Vp-p (19.5 MPa) with a cell viability of 77% after 10 s of insonication. © 2011 IEEE.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14547
https://www.scopus.com/inward/record.uri?eid=2-s2.0-79952934869&doi=10.1109%2fTBME.2010.2089521&partnerID=40&md5=229e84345021a76604100d233fc834b6
ISSN: 189294
Appears in Collections:Scopus 1983-2021

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