Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14528
ชื่อเรื่อง: α-Mangostin induces apoptosis in human chondrosarcoma cells through downregulation of ERK/JNK and Akt signaling pathway
ผู้แต่ง: Krajarng A.
Nakamura Y.
Suksamrarn S.
Watanapokasin R.
Keywords: Akt
Annexins
Anticancer effects
Antitumor property
Apoptosis
Apotosis
Caspase-3
Chondrosarcoma
Chondrosarcoma cells
Cytochrome C
Dose-dependent manner
Down-regulation
Hoechst
Induced apoptosis
MAPK
Mitochondrial dysfunction
Primary bone tumors
Radiation therapy
Signaling pathways
Cell culture
Cell proliferation
Chemotherapy
Drug products
Electrophoresis
Flow cytometry
Mitochondria
Phosphorylation
Signaling
Cell death
antineoplastic agent
mangostin
mitogen activated protein kinase
protein kinase B
stress activated protein kinase
xanthone derivative
apoptosis
article
bone tumor
cell proliferation
chondrosarcoma
drug effect
human
metabolism
pathology
phosphorylation
signal transduction
tumor cell line
Antineoplastic Agents, Phytogenic
Apoptosis
Bone Neoplasms
Cell Line, Tumor
Cell Proliferation
Chondrosarcoma
Extracellular Signal-Regulated MAP Kinases
Humans
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
Phosphorylation
Proto-Oncogene Proteins c-akt
Signal Transduction
Xanthones
Garcinia mangostana
วันที่เผยแพร่: 2011
บทคัดย่อ: Chondrosarcoma is a malignant primary bone tumor that is resistant to chemotherapy and radiation therapy. α-Mangostin, a component of Garcinia mangostana Linn, is a xanthone derivative shown to have antioxidant and antitumor properties. This study is the first to investigate anticancer effects of α-mangostin in the human chondrosarcoma cell line SW1353. We showed that α-mangostin inhibited cell proliferation of SW1353 cells in a time-and dose-dependent manner by using the trypan blue exclusion method. Hoechst 33342 nuclear staining and nucleosomal DNA-gel electrophoresis revealed that α-mangostin could induce nuclear condensation and fragmentation, typically seen in apoptosis. Flow cytometry using Annexin V/PI double staining assessed apoptosis, necrosis and viability. α-Mangostin activated caspase-3,-8,-9 expression, decreased Bcl-2 and increased Bax. This promotes mitochondrial dysfunction, leading to the release of cytochrome c from the mitochondria to the cytoplasm. In addition, total and phosphorylated ERK and JNK were downregulated in α-mangostin-treated SW1353 cells but no changes in p38. α-Mangostin also decreased phosphorylated Akt without altering total Akt. These results suggest that R-mangostin inhinbited cell proliferation and induced apoptosis through downregulation of ERK, JNK and Akt signaling pathway in human chondrosarcoma SW1353 cells. © 2011 American Chemical Society.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14528
https://www.scopus.com/inward/record.uri?eid=2-s2.0-79957964309&doi=10.1021%2fjf200620n&partnerID=40&md5=af355dc0b01deef4980812eb1da92526
ISSN: 218561
Appears in Collections:Scopus 1983-2021

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