Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14504
ชื่อเรื่อง: Diversity of cultivable hydrogen-producing bacteria isolated from agricultural soils, waste water sludge and cow dung
ผู้แต่ง: Kanso S.
Dasri K.
Tingthong S.
Watanapokasin R.Y.
Keywords: 16S rRNA gene
Cow dung
Diversity
Hydrogen producing bacteria
RFLP
Sludge
Bacteria
Bacteriology
Hydrogen
RNA
Sewage
Wastewater
Genes
วันที่เผยแพร่: 2011
บทคัดย่อ: This project aimed to study the diversity of cultivable hydrogen-producing bacteria, isolated from agricultural soils, waste water sludge and cow dung by analyzing 16S rRNA gene. Isolation performed anaerobically on nutrient agar using environmental samples as inoculum yielded 106 pure isolates. These isolates were tested for their capability to produce hydrogen. Then 16S rRNA gene of the 11 isolates having such ability were PCR amplified and subjected to restriction fragment length polymorphism (RFLP) analysis in order to group them into operational taxonomic units (OTUs). RFLP was evaluated for its ability to group the 1500-1600 bp PCR products into OTUs. Isolates were presumptively identified by analysis of partial 16S rRNA gene sequence data. Each OTU was found to be phylogenetically cohesive. The 11 isolates were put into 2 main groups. Group 1 composted of WS-2-2, AS-I-1 and AS-I-2 whose partial 16S rRNA genes showed similarity of 98-99% to members of the genus Paenibacillus. Group 2 composted of WS-4-2, Lao-1-2, WS-7-11, AS-1, AS-4, CD-5 and CD-6 having highest similarity of 98-99% to members of four genera in the family Enterobacteriacea. AS-I-2 which produced the highest amount of hydrogen gas of 2.70 ml had the highest 16S rRNA gene similarity of 99% to Paenibacillus polymixa. © 2010, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14504
https://www.scopus.com/inward/record.uri?eid=2-s2.0-79958145700&doi=10.1016%2fj.ijhydene.2010.07.010&partnerID=40&md5=3d43981655207c3ab141c88bf93032b0
ISSN: 3603199
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.