Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14271
ชื่อเรื่อง: Sodium-dependent uptake of glutamate by novel ApGltS enhanced growth under salt stress of halotolerant cyanobacterium aphanothece halophytica
ผู้แต่ง: Boonburapong B.
Laloknam S.
Yamada N.
Incharoensakdi A.
Takabe T.
Keywords: Amino acid residues
Amino acid sequence
Amino acid uptake
Aphanothece halophytica
Aspartates
Cyanobacterium
E. coli
Enhanced growth
Free amino acids
Glutamate transporters
Glycine betaine
Halotolerant
High salinity
Kinetics studies
Salt stresss
Synechocystis sp. PCC 6803
Transmembrane segments
Escherichia coli
Hardwoods
Salinity measurement
Sodium
Sodium chloride
Transport properties
Amino acids
amino acid transporter
bacterial protein
betaine
cotransporter
Escherichia coli protein
gltS protein, E coli
glutamate transporter
glutamic acid
messenger RNA
recombinant protein
sodium chloride
amino acid sequence
article
biosynthesis
cyanobacterium
drug effect
enzyme specificity
Escherichia coli
genetics
growth, development and aging
kinetics
metabolism
molecular genetics
molecular weight
phylogeny
physiology
protein tertiary structure
salinity
salt tolerance
sequence homology
Amino Acid Sequence
Amino Acid Transport System X-AG
Amino Acid Transport Systems, Acidic
Bacterial Proteins
Betaine
Cyanobacteria
Escherichia coli
Escherichia coli Proteins
Glutamic Acid
Kinetics
Molecular Sequence Data
Molecular Weight
Phylogeny
Protein Structure, Tertiary
Recombinant Proteins
RNA, Messenger
Salinity
Salt-Tolerance
Sequence Homology, Amino Acid
Sodium Chloride
Substrate Specificity
Symporters
Aphanothece halophytica
Cyanobacteria
Escherichia coli
Synechocystis sp. PCC 6803
วันที่เผยแพร่: 2012
บทคัดย่อ: Glutamate is a major free amino acid in cyanobacteria, but its transport properties remain largely unknown. In this study, we found that a halotolerant cyanobacterium, Aphanothece halophytica, contained a sodium dependent glutamate transporter (ApGltS). The deduced amino acid sequence of ApGltS exhibited low homology (18-19% identity) to GltS from Synechocystis sp. PCC 6803 (slr1145) and Escherichia coli. The predicted ApGltS consisted of 476 amino acid residues with a molecular weight of 50,976 Da. As analysed by hydropathy profiling, ApGltS contains 11 transmembrane segments. The ApgltS gene was isolated and expressed in E. coli ME9107, which is deficient in glutamate uptake. ME9107, expressing ApGltS, took up glutamate and its rates increased with increasing concentrations of NaCl. Kinetics studies revealed that ApGltS is a high-affinity glutamate transporter with a Km of about 5μM. The presence of 0.5M NaCl in the assay medium increased Vmax by about 3-fold. Competition experiments revealed that glutamate, glutamine, aspartate, and asparagine inhibited glutamate uptake. The level of mRNA for ApgltS was higher in A. halophytica grown at high salinity. Under high salinity conditions supplemented with glutamate, A. halophytica showed a significant increase in intracellular glycine betaine.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14271
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84866649295&doi=10.1271%2fbbb.120309&partnerID=40&md5=14d9233fe04df6f1dec602021ba4cb79
ISSN: 9168451
Appears in Collections:Scopus 1983-2021

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