Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14155
Title: Selected activities of Citrus Maxima merr. Fruits on human endothelial cells: Enhancing cell migration and delaying cellular aging
Authors: Buachan P.
Chularojmontri L.
Wattanapitayakul S.K.
Keywords: ascorbic acid
aurantiin
flavonoid
gallic acid
hesperidin
messenger RNA
plant extract
2',7'-dichlorodihydrofluorescein diacetate
antioxidant
ascorbic acid
endothelial nitric oxide synthase
flavanone derivative
fluorescein derivative
gallic acid
hesperidin
NOS3 protein, human
plant extract
reactive oxygen metabolite
antioxidant activity
article
cell aging
cell migration
centrifugation
Citrus maxima
controlled study
endothelium cell
fluorescence recovery after photobleaching
high performance liquid chromatography
human
human cell
oxidative stress
polyacrylamide gel electrophoresis
polymerase chain reaction
protein expression
pummelo
reverse transcription polymerase chain reaction
senescence
Western blotting
wound healing
cell aging
cell motion
chemistry
Citrus
drug effects
fruit
genetics
metabolism
umbilical vein endothelial cell
Antioxidants
Ascorbic Acid
Cell Aging
Cell Movement
Citrus
Flavanones
Fluoresceins
Fruit
Gallic Acid
Hesperidin
Human Umbilical Vein Endothelial Cells
Humans
Nitric Oxide Synthase Type III
Plant Extracts
Reactive Oxygen Species
RNA, Messenger
Issue Date: 2014
Abstract: Endothelial injury and damage as well as accumulated reactive oxygen species (ROS) in aging play a significant role in the development of cardiovascular disease (CVD). Recent studies show an association of high citrus fruit intake with a lower risk of CVD and stroke but the mechanisms involved are not fully understood. This study investigated the effects of pummelo (Citrus maxima Merr. var. Tubtim Siam, CM) fruit extract on human umbilical vein endothelial cell (HUVECs) migration and aging. The freeze-dried powder of fruit extract was characterized for antioxidant capacity (FRAP assay) and certain natural antioxidants, including ascorbic acid, gallic acid, hesperidin, and naringin (HPLC). Short-term (48 h) co-cultivation of HUVECs with CM enhanced cell migration as evaluated by a scratch wound assay and Boyden chamber assay. A long-term treatment with CM for 35 days significantly increased HUVEC proliferation capability as indicated by population doubling level (PDL). CM also delayed the onset of aging phenotype shown by senescence-associated β-galactosidase (SA-β-gal) staining. Furthermore, CM was able to attenuate increased ROS levels in aged cells when determined by 2′,7′-dichlorodihydrofluorescein diacetate (DCDHF) while eNOS mRNA expression was increased but the eNOS protein level was not changed. Thus, further in vivo and clinical studies are warranted to support the use of pummelo as a functional fruit for endothelial health and CVD risk reduction. © 2014 by the authors; licensee MDPI, Basel, Switzerland.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14155
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84899134634&doi=10.3390%2fnu6041618&partnerID=40&md5=b6cf9c258d22202b2c1818e3cd32e7a6
ISSN: 20726643
Appears in Collections:Scopus 1983-2021

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