Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14130
ชื่อเรื่อง: Expressional changes of carbamoyl phosphate synthetase and glutamine synthetase in the liver of rat with thioacetamide-induced cirrhosis
ผู้แต่ง: Khunvirojpanich M.
Wattanasirichaigoon S.
Pradidarcheep W.
Keywords: carbamoyl phosphate synthase
glutamate ammonia ligase
thioacetamide
alanine aminotransferase blood level
alkaline phosphatase blood level
animal experiment
animal model
animal tissue
article
aspartate aminotransferase blood level
controlled study
immunohistochemistry
liver cell
liver cirrhosis
liver function test
liver regeneration
male
microscopy
nonhuman
protein expression
rat
Analysis of Variance
Animals
Carbamyl Phosphate
Glutamate-Ammonia Ligase
Liver Cirrhosis, Experimental
Liver Function Tests
Male
Rats
Rats, Wistar
Thioacetamide
วันที่เผยแพร่: 2013
บทคัดย่อ: Background: In order to detoxify ammonia, mammalian livers use carbamoyl phosphate synthetase (CPS) and glutamine synthetase (GS) for conversion into respective non-toxic urea and glutamine. CPS is expressed in the periportal hepatocytes whereas GS is contained in the pericentral hepatocytes. Objective: To examine the expressional changes of CPS and GS in the liver being induced to become cirrhotic by hepatotoxin thioacetamide (TAA). Material and Method: Twenty-five male Wistar rats were divided into 5 groups of 5 animals each. Group 1 was for control. Groups 2 to 5 were treated with 200 mg/kg TAA intraperitoneally three times weekly for 1, 2, 3 and 4 months respectively. The immunohistochemical technique was employed in order to elucidate the expression of CPS and GS in each animal group. Results: As centro-central fibrous bridging developed in the course of TAA treatment, expression of CPS declined dramatically and that of GS was no longer restricted to the pericentral hepatocytes. In month 4, CPS-positive hepatocytes were only found in some regenerative nodules, whereas GS expression became confined to the nodular periphery. Proper CPS staining required tissue fixation in a mixture of methanol, acetone and water (2:2:1 v/v) as opposed to 4% paraformaldehyde. Conclusion: In response to the hepatotoxin TAA, the liver attempts to regenerate by means of conserving persistent CPSpositive hepatocytes and rearranging GS-positive hepatocytes in response to vascular obstruction.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14130
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84876853950&partnerID=40&md5=fda4d34f954f3b0a54efd0e1e836d68e
ISSN: 1252208
Appears in Collections:Scopus 1983-2021

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