Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14089
ชื่อเรื่อง: Terrein induces apoptosis in HeLa human cervical carcinoma cells through p53 and ERK regulation
ผู้แต่ง: Porameesanaporn Y.
Uthaisang-Tanechpongtamb W.
Jarintanan F.
Jongrungruangchok S.
Wongsatayanon B.T.
Keywords: caspase 3
caspase 8
caspase 9
death receptor
hoe 33342
mitogen activated protein kinase
protein Bax
protein bcl 2
protein p21
protein p53
terrein
antineoplastic activity
apoptosis
article
cancer cell
cancer inhibition
cell cycle G0 phase
cell death
cell proliferation
chromatin condensation
controlled study
DNA fragmentation
drug cytotoxicity
drug development
drug mechanism
drug structure
enzyme activity
enzyme substrate
flow cytometry
HeLa cell
human
human cell
IC 50
mitochondrion
priority journal
protein expression
real time polymerase chain reaction
signal transduction
upregulation
uterine cervix carcinoma
Antineoplastic Agents
Apoptosis
Aspergillus
Carcinoma
Cell Proliferation
Cyclopentanes
Female
Gene Expression Regulation, Neoplastic
HeLa Cells
Humans
MAP Kinase Signaling System
Mitochondria
Tumor Suppressor Protein p53
Uterine Cervical Neoplasms
วันที่เผยแพร่: 2013
บทคัดย่อ: Terrein, a fungal metabolite derived from Aspergillus terreus, has been shown to have a variety of biological activities in human cells including inhibition of melanogenesis, as well as anti-inflammatory, antioxidant and anticancer properties. In the present study, terrein was shown to have marked anticancer activity on HeLa human cervical carcinoma cells. Terrein exhibited inhibition of proliferation within the same ranges for other cancer cell types with an IC50 at 0.29 mM. The growth inhibition that induced cell death was via apoptosis mechanisms. Chromatin condensation was observed using the Hoechst 33342 stain, a DNA-specific dye. The increase of DNA fragmentation or the sub-G0 peak was also detected by flow cytometry. The signaling used by terrein to induce apoptosis was via the death-receptor and mitochondrial pathways; the cleavage of specific fluorogenic substrates by caspase-3, -8 and -9 activities are clearly demonstrated. The mitochondria were damaged as demonstrated by the decrease of the red/green ratio of the JC-1 staining and the increase of the Bax/Bcl-2 expression ratio. Further analysis of the upstream signaling by the quantitative real-time polymerase chain reaction showed that p53, p21 and ERK were upregulated which indicates the importance of their roles on terrein signaling. This study is the first to show that terrein has an effect on the anticancer properties in cervical cancer cells by inducing apoptosis through p53 and ERK regulation. Our data may help expand the function of the terrein compound and may also aid in the discovery of new anticancer agents. © 2013 Spandidos Publications Ltd. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14089
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84874728121&doi=10.3892%2for.2013.2288&partnerID=40&md5=1c51a761fb8a19ab3dfa230a4e4bff1d
ISSN: 1021335X
Appears in Collections:Scopus 1983-2021

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