Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/14027
ชื่อเรื่อง: Interphase-FISH screening for eight common rearrangements in pediatric B-cell precursor acute lymphoblastic leukemia
ผู้แต่ง: Hutspardol S.
Pakakasama S.
Kanta K.
Nuntakarn L.
Anurathapan U.
Sirachainan N.
Songdej D.
Sawangpanich R.
Tiyasirichokchai R.
Rerkamnuaychoke B.
Hongeng S.
Keywords: Abelson kinase
breakpoint cluster region protein
immunoglobulin heavy chain
mixed lineage leukemia protein
transcription factor 7 like 1
transcription factor ETV6
transcription factor PAX5
transcription factor PBX1
transcription factor RUNX1
acute lymphoblastic leukemia
article
child
chromosome rearrangement
clinical article
cytogenetics
DNA probe
female
fluorescence in situ hybridization
fusion gene
gene deletion
gene translocation
human
infant
interphase
male
pre B lymphocyte
preschool child
priority journal
school child
screening
ALL
B-cells
FISH
Acute Disease
Adolescent
B-Lymphocytes
Child
Child, Preschool
Female
Genetic Testing
Humans
In Situ Hybridization, Fluorescence
Infant
Interphase
Karyotyping
Male
Oncogene Proteins, Fusion
Pilot Projects
Precursor B-Cell Lymphoblastic Leukemia-Lymphoma
Translocation, Genetic
วันที่เผยแพร่: 2013
บทคัดย่อ: Summary: Introduction: This is the first pilot study to screen multiple common genetic aberrations in B-cell precursor acute lymphoblastic leukemia (BCP-ALL). Methods: Thirty-two children with BCP-ALL were investigated for chromosomal rearrangements using interphase fluorescence in situ hybridization (FISH). Eight common translocations and rearrangements, including ETV6-RUNX1, TCF3-PBX1, BCR-ABL1, ETV6, TCF3, MLL, IGH@, and PAX5, were tested for using dual-color DNA probes. Results: ETV6-RUNX1 was the most frequent translocation detected in 11 children (34.4%). Two patients with BCR-ABL1 (6.3%) and one with TCF3-PBX1 (3.1%) translocations were also observed. Using break-apart probes, 11 children (34.4%) had a positive FISH result for ETV6, two patients for IGH@ (6.3%), one patient for MLL (3.1%), and one patient for PAX5 rearrangements (3.1%). All patients with the ETV6-RUNX1 fusion were also identified by split signals for ETV6. Other abnormalities, including extra copies and deletion of genes, were observed within the range of 3.1-34.4%. Cytogenetics analysis showed a single case each of BCR-ABL1 fusion, MLL, and IGH@ rearrangements (3.1% each). ETV6-RUNX1 fusion and ETV6 split-apart rearrangements were not visible by cytogenetics. Likewise, one each of cases with TCF3-PBX1 fusion and with PAX5 split signal seen by FISH was not visible by cytogenetics. Conclusion: By using 8 FISH probes in conjunction cytogenetics for the detection of common aberrations, interphase FISH enhanced the detection of chromosomal rearrangements in children with BCP-ALL. © 2012 John Wiley & Sons Ltd.
URI: https://ir.swu.ac.th/jspui/handle/123456789/14027
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84880621984&doi=10.1111%2fijlh.12031&partnerID=40&md5=f204742139e63a8d1787436558acbd4b
ISSN: 17515521
Appears in Collections:Scopus 1983-2021

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