Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13890
ชื่อเรื่อง: Aureobasidium pullulans as a source of liamocins (heavy oils) with anticancer activity
ผู้แต่ง: Manitchotpisit P.
Watanapoksin R.
Price N.P.J.
Bischoff K.M.
Tayeh M.
Teeraworawit S.
Kriwong S.
Leathers T.D.
Keywords: Cell culture
Cells
Crude oil
Desorption
Diseases
Escherichia coli
Mass spectrometry
Anti-cancer agents
Aureobasidium pullulans
Liamocins
Phylogeny
Pullulans
Heavy oil production
Aureobasidium pullulans
Enterococcus faecalis
Escherichia coli
Lactobacillus fermentum
Pseudomonas aeruginosa
Staphylococcus aureus
antiinfective agent
antineoplastic agent
biological product
liamocin A1
liamocin B1
mannitol
oil
sugar alcohol
analogs and derivatives
animal
Ascomycetes
Bacteria
cell proliferation
chemical structure
chemistry
Chlorocebus aethiops
classification
drug effects
HeLa cell line
human
metabolism
Neoplasms
pathology
tumor cell line
Vero cell line
Animals
Anti-Bacterial Agents
Antineoplastic Agents
Ascomycota
Bacteria
Biological Products
Cell Line, Tumor
Cell Proliferation
Cercopithecus aethiops
HeLa Cells
Humans
Mannitol
Molecular Structure
Neoplasms
Oils
Sugar Alcohols
Vero Cells
วันที่เผยแพร่: 2014
บทคัดย่อ: Liamocins are structurally unique, heavier-than-water "oils" produced by certain strains of Aureobasidium pullulans. The aim of the current study is to identify new sources of liamocins and evaluate their potential as anticancer agents. Nine strains of A. pullulans from phylogenetic clades 8, 9, and 11 were examined for the first time for production of liamocins. Strains in these clades have only been isolated from tropical environments, and all strains tested here were from various locations in Thailand. Strains RSU 9, RSU 21, and RSU 29, all from clade 11, produced from 7.0 to 8.6 g liamocins/l from medium containing 5 % sucrose. These are the highest yields of liamocins that we have found thus far. These strains also produced from 9.4 to 17 g pullulan/l. The structural identity of liamocins was confirmed by matrix-assisted laser desorption/ionization mass spectrometry; differential spectra were obtained in which the dominant ion was either at about m/z 805.5 or m/z 949.6, consistent with the structure of liamocins. Liamocins from A. pullulans strains RSU 9 and RSU 21 inhibited two human breast cancer cell lines and a human cervical cancer cell line (IC 50 values of 32.2 ± 1.4 to 63.1 ± 2.4 μg liamocins/ml) but were not toxic to a normal cell line. Liamocins weakly inhibited a strain of Enterococcus faecalis, but did not inhibit strains of Lactobacillus fermentum, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Thus, A. pullulans phylogenetic clade 11 is a promising source of liamocins, and these compounds merit further examination as potential anticancer agents. © 2014 Springer Science+Business Media Dordrecht (outside the USA).
URI: https://ir.swu.ac.th/jspui/handle/123456789/13890
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84903527305&doi=10.1007%2fs11274-014-1639-7&partnerID=40&md5=499ce9f8639d7713fcccec143cafd52f
ISSN: 9593993
Appears in Collections:Scopus 1983-2021

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