Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13880
ชื่อเรื่อง: Type I and type II crustins from Penaeus monodon, genetic variation and antimicrobial activity of the most abundant crustinPm4
ผู้แต่ง: Donpudsa S.
Visetnan S.
Supungul P.
Tang S.
Tassanakajon A.
Rimphanitchayakit V.
Keywords: humoral antibody
kanamycin
recombinant crustinPm4 1 protein
recombinant protein
thioredoxin
type 1 crustin
type 2 crustin
unclassified drug
antimicrobial cationic peptide
arthropod protein
amino terminal sequence
animal cell
animal experiment
animal tissue
antimicrobial activity
antiviral activity
article
Bacillus megaterium
Bacillus subtilis
bacterial growth
blood cell
controlled study
DNA virus infection
drug effect
Escherichia coli
gene expression regulation
genetic variability
growth inhibition
LD 50
Micrococcus luteus
nonhuman
nucleotide sequence
Penaeus monodon
priority journal
protein expression
sequence analysis
Staphylococcus aureus
upregulation
Vibrio harveyi
White spot syndrome virus
amino acid sequence
animal
cell culture
chemistry
genetic variability
genetics
immunology
microbiology
molecular genetics
Penaeidae
sequence alignment
virology
Amino Acid Sequence
Animals
Antimicrobial Cationic Peptides
Arthropod Proteins
Cells, Cultured
Genetic Variation
Hemocytes
Molecular Sequence Data
Penaeidae
Sequence Alignment
วันที่เผยแพร่: 2014
บทคัดย่อ: An antimicrobial protein, crustin, is involved in the innate immunity of crustacean by defending the host directly against the microbial pathogens. By data mining the Penaeus monodon EST database, two type I crustins, carcinin. Pm1 and 2, and ten type II crustins, crustin. Pm1-10, were identified. The abundant crustins were crustin. Pm1, 4 and 7, each with variation in the length of Gly-rich repeat among its members. A few crustin. Pm1, 4 and 7 with deletion in the Cys-rich region were also observed. Furthermore, the crustin. Pm4 with the longest N-terminal Gly-rich region was characterized. The crustinPm4 allelic genes were expressed mainly from the hemocytes. Its expression was up-regulated readily by WSSV infection and gradually decreased to normal level afterwards. The recombinant crustin. Pm4-1 (rcrustin. Pm4-1) isoform was produced using the Escherichia coli expression system and tested for its antimicrobial activity. The rcrustin. Pm4-1 was able to inhibit the growth of a Gram-positive bacterium, Bacillus megaterium but not Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus. It also inhibited the growth of two Gram-negative bacteria, E. coli 363 and Vibrio harveyi 639 at lower potency. The rcrustin. Pm4-1 affected the WSSV infection because the expression of an intermediate early gene ie1 in WSSV-infected hemocyte cell culture was reduced. It was shown further that the rcrustin. Pm4-1 could delay by about one and a half days the manifestation of disease by WSSV. © 2014 Elsevier Ltd.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13880
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84904880634&doi=10.1016%2fj.dci.2014.06.015&partnerID=40&md5=c7058ef3605abcd19f09aefb0032cf6d
ISSN: 0145305X
Appears in Collections:Scopus 1983-2021

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