Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13856
ชื่อเรื่อง: Molecular cloning and characterization of a Toll receptor gene from Macrobrachium rosenbergii
ผู้แต่ง: Srisuk C.
Longyant S.
Senapin S.
Sithigorngul P.
Chaivisuthangkura P.
Keywords: arthropod protein
toll like receptor
Aeromonas punctata
amino acid sequence
animal
article
chemistry
classification
gene expression regulation
genetics
immunology
innate immunity
Macrobrachium rosenbergii
metabolism
microbiology
molecular genetics
nucleotide sequence
Palaemonidae
phylogeny
physiology
RNA interference
sequence alignment
Toll receptor
Aeromonas caviae
Innate immunity
Macrobrachium rosenbergii
RNA interference
Toll receptor
Aeromonas caviae
Amino Acid Sequence
Animals
Arthropod Proteins
Base Sequence
Gene Expression Regulation
Immunity, Innate
Molecular Sequence Data
Palaemonidae
Phylogeny
RNA Interference
Sequence Alignment
Toll-Like Receptors
วันที่เผยแพร่: 2014
บทคัดย่อ: Toll receptors are cell surface molecules acting as pattern recognition receptors (PRRs) that have been implicated in the signaling pathway of innate immune responses. In this study, the full-length cDNA of a Toll receptor gene of Macrobrachium rosenbergii, designated MrToll, was successfully isolated using designed degenerate primers and the rapid amplification of cDNA ends (RACE). The MrToll gene sequence contained an open reading frame (ORF) of 2799 nucleotides encoding a protein of 932 amino acid residues. The protein contained distinct structural motifs of the Toll-like receptor (TLR) family, including an extracellular domain containing 15 leucine-rich repeats (LRRs), a transmembrane segment of 23 amino acids, and a cytoplasmic Toll/interleukin-1R (TIR) domain of 139 residues. Phylogenetic analysis revealed that MrToll and Toll receptor of Marsupenaeus japonicus (MjToll) evolved closely. However, the MrToll ORF demonstrated only 48-49% identity with shrimp Toll1, suggesting that MrToll isolated from a palaemonid shrimp might belong to a novel class of Toll receptors in shrimp. The transcripts of the MrToll gene were constitutively expressed in various tissues, with high levels in hemocytes, the stomach and muscle. A reverse transcriptase PCR assay demonstrated that the expression patterns of MrToll were distinctly modulated after Aeromonas caviae stimulation, with significant enhancement at 3-12h post-challenge and a decline to basal levels at 24h post-challenge. In addition, when MrToll-silenced shrimp were challenged with A.caviae, there was a significant increase in mortality and bacterial CFU counts. These results suggest that MrToll might be involved in host innate defense, especially against the pathogen A.caviae. © 2014 Elsevier Ltd.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13856
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84893204687&doi=10.1016%2fj.fsi.2013.12.025&partnerID=40&md5=ce291f846686d59f45c1a732d91f395f
ISSN: 10504648
Appears in Collections:Scopus 1983-2021

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