Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13778
ชื่อเรื่อง: In vitro screening of lactic acid bacteria for multi-strain probiotics
ผู้แต่ง: Pringsulaka O.
Rueangyotchanthana K.
Suwannasai N.
Watanapokasin R.
Amnueysit P.
Sunthornthummas S.
Sukkhum S.
Sarawaneeyaruk S.
Rangsiruji A.
Keywords: Anas
Animalia
Escherichia coli
Gallus gallus
Lactobacillus paraplantarum
Lactobacillus plantarum
Lactobacillus reuteri
Salmonella sp.
Shigella sp.
Staphylococcus aureus
Suidae
วันที่เผยแพร่: 2015
บทคัดย่อ: The aim of this research was to screen and evaluate some probiotic properties of lactic acid bacteria strains isolated from 150 animal fecal samples, such as cows, pigs, chickens, and ducks. To achieve an objective, 81 isolates were tested for antimicrobial activity, tolerance to bile salts and acid, potential adhesion to cell surface, and in vitro adhesion. The results of the antimicrobial activity against pathogenic bacteria showed that, out of 81 isolates, 61, 59, 43, 78, and 79 isolates had antimicrobial activity against Escherichia coli, Staphylococcus aureus, Salmonella sp., Shigella sp., and Kiebsiella sp., respectively. Most lactic acid bacteria (LAB) isolates were tolerant to 1.0% bile salts and were able to survive at pH 3.5 for 3h. Twelve isolates were then evaluated on their hydrophobic character. Five isolates with probiotic properties were subsequently selected. Sequence analysis of 16S rDNA demonstrated that 2 isolates belong to Lactobacillus reuteri (strains P8 and P30), 2 to Lactobacillus plantarum (strains P6 and P31), and another one to Lactobacillus paraplantarum (strain P25). To assess their viability in broiler chicken diets, 3 strains, L. plantarum (strain P6), L. paraplantarum (strain P25), and L. reuteri (strain P30), containing 108cfu/g were inoculated into a commercial chicken diet in the form of single strain and multi-strain preparations. We found that in all of the treatments, the numbers of viable cells of LAB (cfu/g of diet) were decreased below 107cfu/g after 3d of incubation. Therefore, based on our study, the single- and multi-strains may have a potential use as novel probiotics in broiler chicken diets. © 2015 Elsevier B.V.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13778
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925214488&doi=10.1016%2fj.livsci.2015.01.016&partnerID=40&md5=68da4a7edb3bfefb9b5778092cfa6afa
ISSN: 18711413
Appears in Collections:Scopus 1983-2021

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