Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13719
ชื่อเรื่อง: Soy isoflavones improves endometrial barrier through tight junction gene expression
ผู้แต่ง: Kiatprasert P.
Deachapunya C.
Benjanirat C.
Poonyachoti S.
Keywords: claudin 1
claudin 3
claudin 4
claudin 7
claudin 8
daidzein
endotoxin
fluorescein isothiocyanate dextran
genistein
isoflavone derivative
lipopolysaccharide
messenger RNA
protein ZO1
tight junction protein
claudin
genistein
isoflavone derivative
lipopolysaccharide
phytoestrogen
protein ZO1
animal cell
Article
concentration response
controlled study
electric resistance
endometrium
female
gene expression
genetic transcription
monolayer culture
nonhuman
priority journal
real time polymerase chain reaction
soybean
swine
tight junction
transepithelial electrical resistance
animal
cell line
drug effects
endometrium
gene expression
genetics
metabolism
pig
tight junction
Bacteria (microorganisms)
Sus
Animals
Cell Line
Claudins
Endometrium
Female
Gene Expression
Genistein
Isoflavones
Lipopolysaccharides
Phytoestrogens
Swine
Tight Junctions
Zonula Occludens-1 Protein
วันที่เผยแพร่: 2015
บทคัดย่อ: Contamination with bacterial endotoxin causes the disruption of the tight junction (TJ) barrier. We investigated the ameliorative effect of dietary flavonoids genistein (Ge) and daidzein (Di) in normal or lipopolysaccharide (LPS)-induced disruption of epithelial barrier function of the endometrium. Using the immortalized porcine glandular endometrial epithelial cells (PEG), transepithelial electrical resistance (TER) and FITC-dextran flux (FD-4) across the monolayer were measured. The mRNA expression of TJ proteins, zona occludens-1 (ZO1), and claudin-1, -3, -4, -7 and -8 was evaluated by real-time RT-PCR for coinciding effect of Ge or Di occurred at the gene transcription level. The results revealed that Ge and Di altered the TER, depending on times and concentrations. Low concentration (10K10 M) of both compounds decreased the TER, whereas higher concentrations (10-8 and 10-6 M) increased the TER which was not related to the FD-4 flux. The increased TER by Ge or Di was parallel to the induction of claudin-3 and -4 or -8 mRNA expression respectively. With LPS inoculation, all isoflavone treatments inhibited the decreased TER induced by LPS, but only Ge (10-8 or 10-6 M) or Di (10-10 or 10-6 M) was coincidence with the decreased FD-4 flux. Under this LPS-stimulated condition, some or all examined TJ gene expressions appeared to be promoted by specific concentration of Ge or Di respectively. Our findings suggest that the soy isoflavones treatment could promote and restore the impaired endometrial barrier function caused by LPS contamination. © 2015 Society for Reproduction and Fertility.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13719
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84922355352&doi=10.1530%2fREP-14-0269&partnerID=40&md5=3f1743726355b718931365ede3d79f6d
ISSN: 14701626
Appears in Collections:Scopus 1983-2021

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