Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13650
ชื่อเรื่อง: Alpha-mangostin partially preserves expression of ammonia-metabolizing enzymes in thioacetamide- induced fibrotic and cirrhotic rats
ผู้แต่ง: Khunvirojpanich M.
Showpittapornchai U.
Moongkarndi P.
Pradidarcheep W.
Keywords: alpha mangostin
carbamoyl phosphate synthase
glutamate ammonia ligase
thioacetamide
unclassified drug
xanthone derivative
ammonia
glutamate ammonia ligase
mangostin
xanthone derivative
animal experiment
animal model
animal tissue
Article
central vein
controlled study
histology
immunohistochemistry
immunoreactivity
liver cell
liver cirrhosis
liver nodule
liver protection
male
neuroectoderm
nonhuman
perineural vascular plexus
prevention and control
protein expression
rat
thioacetamide-induced liver fibrosis
tissue fixation
animal
liver cirrhosis
metabolism
Wistar rat
Ammonia
Animals
Glutamate-Ammonia Ligase
Hepatocytes
Liver Cirrhosis
Male
Rats
Rats, Wistar
Thioacetamide
Xanthones
วันที่เผยแพร่: 2015
บทคัดย่อ: Background: Ammonia metabolizing enzymes, carbamyol phosphate synthetase (CPS) and glutamine synthetase (GS), are expressed in the periportal and pericentral hepatocytes, respectively. CPS and GS function complementary to ensure complete ammonia detoxification. Immunohistochemical analysis confirmed the decline of both CPS and GS in cirrhotic rat liver induced by thioacetamide (TAA). Alpha-mangostin (AM), a major derivative of xanthone from mangosteen, has been reported to possess a wide range of pharmacological properties. Objective: To examine the preventive effects of AM on CPS and GS expression in fibrotic and cirrhotic rats induced by TAA over sixteen weeks. Material and Method: Twenty-four male Wistar rats were divided into 4 groups of 6 animals each. Group 1 was for control. Group 2 was for pure TAA treatment. Group 3 was for pure AM administration. Group 4, prevention group, was concurrently treated with TAA and AM. Immunohistochemical technique was employed in order to elucidate the expression of CPS and GS in each animal group. Results: Immunohistochemical staining for CPS and GS showed an increasing decline from week eight to sixteen under pure- TAA condition. Fibrous bridgings, nodule formations, and regenerative nodules were detected. Pure-AM condition yielded strongly CPS and GS-stained hepatocytes in a fashion similar to the control. Results from the prevention group showed a decreasing decline of CPS and GS immuno-reactivity from week eight to sixteen as compared to pure-TAA condition. Fewer fibrous portal-caval bridgings were observed at week eight and CPS-positive hepatocytes were found in continuous rings. Conclusion: Alpha-mangostin could partially preserve the normal expression of ammonia-metabolizing enzymes under TAA-induced fibrotic and cirrhotic conditions. © 2015, Medical Association of Thailand. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13650
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957676182&partnerID=40&md5=8beb2ff0f8ac20f12ae8b418bfc2ade9
ISSN: 1252208
Appears in Collections:Scopus 1983-2021

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