Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13491
ชื่อเรื่อง: Application of surface plasmon resonance biosensor for the detection of Candida albicans
ผู้แต่ง: Yodmongkol S.
Thaweboon S.
Thaweboon B.
Puttharugsa C.
Sutapun B.
Amarit R.
Somboonkaew A.
Srikhirin T.
Keywords: Antibodies
Bacteria
Biosensors
Candida
Chemical detection
Escherichia coli
Plasmons
Signal detection
Yeast
11-mercaptoundecanoic acid
Detection limits
Direct detection
Lactobacillus casei
Self-assemble monolayers
Staphylococcus aureus
Surface plasmon resonance biosensor
Surface plasmon resonance imaging
Surface plasmon resonance
วันที่เผยแพร่: 2016
บทคัดย่อ: In this study, surface plasmon resonance imaging (SPR imaging) was developed for the detection of Candida albicans which is a causal agent of oral infection. The detection was based on the sandwich assay. The capture antibody was covalently immobilized on the mixed self assemble monolayers (SAMs). The ratio of mixed SAMs between 11-mercaptoundecanoic acid and 3-mercaptopropanol was varied to find the optimal ratio for use as a sensor surface. The results showed that the suitable surface for C. albicans detection was SAM of carboxylic (mixed SAMs 1 : 0), even though mixed SAMs 1 : 40 had a high detection signal in comparison to mixed SAMs 1 : 0, but the non-specific signal was higher. The detection limit was 107 cells/ml for direct detection, and was increased to 106 cells/ml with sandwich antibody. The use of polyclonal C. albicans antibody as capture and sandwich antibody showed good selectivity against the relevant oral bacteria including Escherichia coli, Streptococcus mutan, Staphylococcus aureus, β-streptococci, and Lactobacillus casei. SPR platform in this study could detect C. albicans from the mixed microbial suspension without requirement of skillful technician. This SPR imaging biosensor could be applied for Candida identification after cultivation. © 2016 The Japan Society of Applied Physics.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13491
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84956625420&doi=10.7567%2fJJAP.55.02BE03&partnerID=40&md5=3470acc7a6bb84227318002263155fcc
ISSN: 214922
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.