Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13483
ชื่อเรื่อง: Accumulation of gamma-aminobutyric acid in the halotolerant cyanobacterium Aphanothece halophytica under salt and acid stress
ผู้แต่ง: Boonburapong B.
Laloknam S.
Incharoensakdi A.
วันที่เผยแพร่: 2016
บทคัดย่อ: γ-Aminobutyric acid (GABA) is known as an inhibitory neurotransmitter in human, while in plants, GABA is an intermediate for amino acid metabolism and also is accumulated in response to a wide range of environmental stress. In the present study, GABA accumulation in Aphanothece halophytica was increased 2-fold in mid-log phase cells grown under salt stress (2.0 M NaCl). When mid-log phase cells were subjected to changes in NaCl concentrations and pH for 4 h, the highest GABA accumulation was observed in cells adapted in medium that contained 2.0 M NaCl and that was adjusted to pH 4.0, respectively. The increase of GABA accumulation was accompanied by an increased glutamate decarboxylase activity. Addition of glutamate to growth medium stimulated GABA accumulation under acid stress but had no effect under salt stress. However, the highest GABA accumulation was detected in cells exposed to both high salt and acid stresses combined with the 5 mM glutamate supplementation with an approximately 3-fold increase as compared to the control. The unicellular A. halophytica showed a similarly high content of GABA to that of a filamentous Arthrospira platensis suggesting the possibility of genetic manipulation of the genes of A. halophytica involved in GABA synthesis to increase GABA yield. © 2015, Springer Science+Business Media Dordrecht.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13483
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84957427848&doi=10.1007%2fs10811-015-0523-7&partnerID=40&md5=74a279e87f54e579849cfe7e16b48760
ISSN: 9218971
Appears in Collections:Scopus 1983-2021

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