Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13477
ชื่อเรื่อง: Heterologous expression and antimicrobial activity of OsGASR3 from rice (Oryza sativa L.)
ผู้แต่ง: Boonpa K.
Tantong S.
Weerawanich K.
Panpetch P.
Pringsulaka O.
Yingchutrakul Y.
Roytrakul S.
Sirikantaramas S.
Keywords: plant protein
Fusarium
gene expression regulation
genetics
Helminthosporium
metabolism
microbiology
Oryza
physiology
plant disease
Xanthomonas campestris
Fusarium
Gene Expression Regulation, Plant
Helminthosporium
Oryza
Plant Diseases
Plant Proteins
Xanthomonas campestris
วันที่เผยแพร่: 2018
บทคัดย่อ: According to an in silico analysis, OsGASR3 (LOC_Os03g55290) from rice (Oryza sativa L.) was predicted to be involved in plant defense mechanisms. A semi-quantitative reverse transcription polymerase chain reaction assay revealed that OsGASR3 is highly expressed in the inflorescences of Thai jasmine rice (O. sativa L. subsp. indica ‘KDML 105′). To characterize the biological activity of OsGASR3, we produced an OsGASR3–glutathione S-transferase fusion protein in Escherichia coli Rosetta-gami (DE3) cells for a final purified recombinant OsGASR3 yield of 0.65 mg/L. The purified OsGASR3 inhibited the hyphal growth of Fusarium oxysporum f.sp. cubense and Helminthosporium oryzae at a relatively low concentration (7.5 μg/mL). Furthermore, OsGASR3 exhibited in planta inhibitory activity against Xanthomonas campestris, suggesting its involvement in defense mechanisms, in addition to its previously reported functions affecting growth and development. These observations indicate that recombinant OsGASR3 may be useful for protecting agriculturally important crops against pathogenic microbes. © 2018 Elsevier GmbH
URI: https://ir.swu.ac.th/jspui/handle/123456789/13477
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85044599217&doi=10.1016%2fj.jplph.2018.03.013&partnerID=40&md5=e5f2f5b5c27e95870641a055c242733c
ISSN: 1761617
Appears in Collections:Scopus 1983-2021

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