Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13474
ชื่อเรื่อง: Proof-of concept that an acute trophic factors intervention after spinal cord injury provides an adequate niche for neuroprotection, recruitment of nestin-expressing progenitors and regeneration
ผู้แต่ง: Krityakiarana W.
Zhao P.M.
Nguyen K.
Gomez-Pinilla F.
Kotchabhakdi N.
De Vellis J.
Espinosa-Jeffrey A.
Keywords: brain derived neurotrophic factor
epidermal growth factor receptor
glial cell line derived neurotrophic factor
green fluorescent protein
heat shock protein 70
heme oxygenase 1
hybrid protein
intermediate filament protein
nestin
recombinant growth factor
somatomedin C
transferrin
TSC1
unclassified drug
nestin
neuroprotective agent
animal cell
animal experiment
animal model
animal tissue
Article
axon
cell count
cell regeneration
controlled study
cytoarchitecture
demyelination
early intervention
gray matter
immunohistochemistry
mitochondrion
motoneuron
mouse
neural stem cell
neuroprotection
nonhuman
oxidative stress
priority journal
protein expression
protein phosphorylation
remyelinization
spinal cord
spinal cord injury
stem cell niche
Wallerian degeneration
white matter
animal
metabolism
pathophysiology
spinal cord injury
stem cell
transgenic mouse
Animals
Mice
Mice, Transgenic
Nestin
Neuroprotective Agents
Spinal Cord Injuries
Stem Cells
วันที่เผยแพร่: 2016
บทคัดย่อ: Trophic factor treatment has been shown to improve the recovery of brain and spinal cord injury (SCI). In this study, we examined the effects of TSC1 (a combination of insulin-like growth factor 1 and transferrin) 4 and 8 h after SCI at the thoracic segment level (T12) in nestin- GFP transgenic mice. TSC1 treatment for 4 and 8 h increased the number of nestin-expressing cells around the lesion site and prevented Wallerian degeneration. Treatment with TSC1 for 4 h significantly increased heat shock protein (HSP)-32 and HSP-70 expression 1 and 2 mm from lesion site (both, caudal and rostral). Conversely, the number of HSP-32 positive cells decreased after an 8-h TSC1 treatment, although it was still higher than in both, non-treated SCI and intact spinal cord animals. Furthermore, TSC1 increased NG2 expressing cell numbers and preserved most axons intact, facilitating remyelination and repair. These results support our hypothesis that TSC1 is an effective treatment for cell and tissue neuroprotection after SCI. An early intervention is crucial to prevent secondary damage of the injured SC and, in particular, to prevent Wallerian degeneration. © Springer Science+Business Media New York 2016.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13474
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84958751578&doi=10.1007%2fs11064-016-1850-z&partnerID=40&md5=8d3d558d572c97d4921f430836c45792
ISSN: 3643190
Appears in Collections:Scopus 1983-2021

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