Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13412
ชื่อเรื่อง: Effects of intramuscular administration of 1α,25(OH)2D3 during skeletal muscle regeneration on regenerative capacity, muscular fibrosis, and angiogenesis
ผู้แต่ง: Srikuea R.
Hirunsai M.
Keywords: calcitriol receptor
colecalciferol
myogenin
myosin heavy chain
animal
C57BL mouse
cell differentiation
drug effects
fibrosis
intramuscular drug administration
male
metabolism
morphogenesis
mouse
muscle disease
neovascularization (pathology)
procedures
regeneration
skeletal muscle
wound healing
Animals
Cell Differentiation
Cholecalciferol
Fibrosis
Injections, Intramuscular
Male
Mice
Mice, Inbred C57BL
Morphogenesis
Muscle, Skeletal
Muscular Diseases
Myogenin
Myosin Heavy Chains
Neovascularization, Pathologic
Receptors, Calcitriol
Regeneration
Wound Healing
วันที่เผยแพร่: 2016
บทคัดย่อ: The recent discovery of the vitaminDreceptor (VDR) in regenerating muscle raises the question regarding the action of Vitamin D3 on skeletal muscle regeneration. To investigate the action of Vitamin D3 on this process, the tibialis anterior muscle of male C57BL/6 mice (10 wk of age) was injected with 1.2% BaCl2 to induce extensive muscle injury. The bioactive form of Vitamin D3 [1α,25(OH)2D3] was administered daily via intramuscular injections during the regenerative phase (days 4-7 postinjury). Physiological and supraphysiological doses of 1α,25(OH)2D3 relative to 1 αg/kg muscle wet weight and mouse body weight were investigated. Muscle samples were collected on day 8 postinjury to examine proteins related to Vitamin D3 metabolism (VDR, CYP24A1, and CYP27B1), satellite cell differentiation and regenerative muscle fiber formation [myogenin and embryonic myosin heavy chain (EbMHC)], protein synthesis signaling (Akt, p70 S6K1, 4E-BP1, and myostatin), fiber-Type composition (fast and slow MHCs), fibrous formation (vimentin), and angiogenesis (CD31). Administration of 1α,25(OH)2D3 at physiological and supraphysiological doses enhanced VDR expression in regenerative muscle. Moreover, CYP24A1 and vimentin expression was increased, accompanying decreased myogenin and EbMHC expression at the supraphysiological dose. However, there was no change in CYP27B1, Akt, p70 S6K1, 4E-BP1, myostatin, fast and slow MHCs, or CD31 expression at any dose investigated. Taken together, administration of 1α,25(OH)2D3 at a supraphysiological dose decreased satellite cell differentiation, delayed regenerative muscle fiber formation, and increased muscular fibrosis. However, protein synthesis signaling, fiber-Type composition, and angiogenesis were not affected by either 1α,25(OH)2D3 administration at a physiological or supraphysiological dose. © 2016 the American Physiological Society.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13412
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983604708&doi=10.1152%2fjapplphysiol.01018.2015&partnerID=40&md5=27c8b6eca6a56731ea580b4353f1b2f2
ISSN: 87507587
Appears in Collections:Scopus 1983-2021

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