Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13289
ชื่อเรื่อง: CXCR4-targeted nanoparticles reduce cell viability, induce apoptosis and inhibit SDF-1α induced BT-549-Luc cell migration in vitro
ผู้แต่ง: Chittasupho C.
Kewsuwan P.
Murakami T.
Keywords: chemokine
chemokine receptor CXCR4 antagonist
cytotoxic agent
doxorubicin
lfc 131
nanoparticle
polyglactin
stromal cell derived factor 1alpha
tyrosinylarginylarginine sodium iodide glycine
unclassified drug
antineoplastic antibiotic
chemokine receptor CXCR4
CXCL12 protein, human
CXCR4 protein, human
doxorubicin
lactic acid
ligand
nanoparticle
oligopeptide
polyglycolic acid
polylactic acid-polyglycolic acid copolymer
stromal cell derived factor 1
apoptosis
Article
breast cancer cell line
BT 549 Luc cell line
cancer chemotherapy
cell migration
cell viability
comparative study
controlled study
dose response
drug delivery system
drug efficacy
drug protein binding
human
human cell
in vitro study
incubation time
internalization
MTT assay
priority journal
antagonists and inhibitors
apoptosis
breast tumor
cell motion
cell proliferation
cell survival
chemistry
drug effect
drug screening
metabolism
pathology
tumor cell culture
Antibiotics, Antineoplastic
Apoptosis
Breast Neoplasms
Cell Movement
Cell Proliferation
Cell Survival
Chemokine CXCL12
Doxorubicin
Drug Screening Assays, Antitumor
Humans
Lactic Acid
Ligands
Nanoparticles
Oligopeptides
Polyglycolic Acid
Receptors, CXCR4
Tumor Cells, Cultured
วันที่เผยแพร่: 2017
บทคัดย่อ: Background: CXCR4 possesses a critical role in several intracellular events such as chemotaxis, invasion and adhesion, which are associated with metastasis of cancer cell. Objective: In this study, CXCR4 targeted polymeric nanoparticle was developed for delivering cytotoxic drug and blocking the chemokine induced migration of cells expressing CXCR4. Method: A peptide which was a linear form of CXCR4 antagonist (LFC131) was attached to PLGA nanoparticles (LFC131-NPs) and PLGA nanoparticles encapsulating DOX (LFC131-DOX-NPs). The cellular binding and internalization of LFC131-DOX-NPs were investigated. Results: The binding and internalization of LFC131-DOX-NPs were higher and more rapidly compared to unconjugated NPs. LFC131-NPs blocked SDF-1α induced migration of BT-549-Luc cells. MTT assays demonstrated that LFC131-NPs and LFC131-DOX-NPs decreased cell viability in a dose dependent manner in 24, 72 and 120 h incubation. Conclusion: A treatment concept of blocking breast cancer cell migration from interaction with SDF- 1α by using LFC131-NPs and then attacking breast cancer cells with doxorubicin might increase the efficacy of current breast cancer treatment. © 2017 Bentham Science Publishers.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13289
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041060602&doi=10.2174%2f1567201814666170216130448&partnerID=40&md5=cfe5aa097f88c55457c776568f239402
ISSN: 15672018
Appears in Collections:Scopus 1983-2021

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