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https://ir.swu.ac.th/jspui/handle/123456789/13289| Title: | CXCR4-targeted nanoparticles reduce cell viability, induce apoptosis and inhibit SDF-1α induced BT-549-Luc cell migration in vitro |
| Authors: | Chittasupho C. Kewsuwan P. Murakami T. |
| Keywords: | chemokine chemokine receptor CXCR4 antagonist cytotoxic agent doxorubicin lfc 131 nanoparticle polyglactin stromal cell derived factor 1alpha tyrosinylarginylarginine sodium iodide glycine unclassified drug antineoplastic antibiotic chemokine receptor CXCR4 CXCL12 protein, human CXCR4 protein, human doxorubicin lactic acid ligand nanoparticle oligopeptide polyglycolic acid polylactic acid-polyglycolic acid copolymer stromal cell derived factor 1 apoptosis Article breast cancer cell line BT 549 Luc cell line cancer chemotherapy cell migration cell viability comparative study controlled study dose response drug delivery system drug efficacy drug protein binding human human cell in vitro study incubation time internalization MTT assay priority journal antagonists and inhibitors apoptosis breast tumor cell motion cell proliferation cell survival chemistry drug effect drug screening metabolism pathology tumor cell culture Antibiotics, Antineoplastic Apoptosis Breast Neoplasms Cell Movement Cell Proliferation Cell Survival Chemokine CXCL12 Doxorubicin Drug Screening Assays, Antitumor Humans Lactic Acid Ligands Nanoparticles Oligopeptides Polyglycolic Acid Receptors, CXCR4 Tumor Cells, Cultured |
| Issue Date: | 2017 |
| Abstract: | Background: CXCR4 possesses a critical role in several intracellular events such as chemotaxis, invasion and adhesion, which are associated with metastasis of cancer cell. Objective: In this study, CXCR4 targeted polymeric nanoparticle was developed for delivering cytotoxic drug and blocking the chemokine induced migration of cells expressing CXCR4. Method: A peptide which was a linear form of CXCR4 antagonist (LFC131) was attached to PLGA nanoparticles (LFC131-NPs) and PLGA nanoparticles encapsulating DOX (LFC131-DOX-NPs). The cellular binding and internalization of LFC131-DOX-NPs were investigated. Results: The binding and internalization of LFC131-DOX-NPs were higher and more rapidly compared to unconjugated NPs. LFC131-NPs blocked SDF-1α induced migration of BT-549-Luc cells. MTT assays demonstrated that LFC131-NPs and LFC131-DOX-NPs decreased cell viability in a dose dependent manner in 24, 72 and 120 h incubation. Conclusion: A treatment concept of blocking breast cancer cell migration from interaction with SDF- 1α by using LFC131-NPs and then attacking breast cancer cells with doxorubicin might increase the efficacy of current breast cancer treatment. © 2017 Bentham Science Publishers. |
| URI: | https://ir.swu.ac.th/jspui/handle/123456789/13289 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85041060602&doi=10.2174%2f1567201814666170216130448&partnerID=40&md5=cfe5aa097f88c55457c776568f239402 |
| ISSN: | 15672018 |
| Appears in Collections: | Scopus 1983-2021 |
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