Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13177
ชื่อเรื่อง: P38 inhibitor inhibits the apoptosis of cowanin-treated human colorectal adenocarcinoma cells
ผู้แต่ง: Chowchaikong N.
Nilwarangkoon S.
Laphookhieo S.
Tanunyutthawongse C.
Watanapokasin R.
Keywords: antineoplastic agent
caspase 7
caspase 9
cowanin
hoe 33342
mitogen activated protein kinase
mitogen activated protein kinase p38 inhibitor
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
phosphoinositide dependent protein kinase 1
protein Bax
protein bcl 2
protein kinase B
unclassified drug
xanthone derivative
4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole
CASP7 protein, human
CASP9 protein, human
caspase 7
caspase 9
cowaniin
imidazole derivative
mitogen activated protein kinase p38
nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
protein kinase B
pyridine derivative
tannin derivative
Akt signaling
antiproliferative activity
apoptosis
Article
cell death
cell nucleus
cell structure
cell survival
cell viability
controlled study
drug cytotoxicity
drug mechanism
drug structure
human
human cell
LoVo cell line
mitochondrial membrane potential
MTT assay
priority journal
protein expression level
protein synthesis
Western blotting
antagonists and inhibitors
cell cycle
cell proliferation
chemistry
colorectal tumor
drug effect
gene expression regulation
MAPK signaling
metabolism
tumor cell line
Caspase 7
Caspase 9
Cell Cycle
Cell Line, Tumor
Cell Proliferation
Cell Survival
Colorectal Neoplasms
Gene Expression Regulation, Neoplastic
Humans
Imidazoles
MAP Kinase Signaling System
p38 Mitogen-Activated Protein Kinases
Poly(ADP-ribose) Polymerases
Proto-Oncogene Proteins c-akt
Pyridines
Tannins
วันที่เผยแพร่: 2018
บทคัดย่อ: Colorectal cancer, which is the third most common type of cancer diagnosed in both men and women, is the leading cause of cancer-related deaths worldwide. Cowanin is a pure compound extracted from Garcinia cowa Roxb., a tree species present in Thailand, Malaysia and Myanmar. The crude extract has been demonstrated to have antitumor activity, inflammation induction, antibacterial activity, anti-inflammatory activity and antimalarial activity. In the present study, the effects of cowanin on apoptosis induction and on the apoptosis- related and mitogen-Activated protein kinase (MAPK) pathways were investigated in the LoVo human colorectal cancer cell line. The cytotoxicity of cowanin in LoVo cells was determined by MTT assay. Hoechst 33342 and JC-1 staining were used to determine nuclear morphological changes and mitochondrial membrane potential, respectively. The expression levels of BCL2 apoptosis regulator (Bcl-2) family, MAPK and AKT serine/threonine kinase 1 (Akt) pathway proteins following cowanin treatment were determined by western blot analysis. The results demonstrated that cowanin inhibited cell proliferation and induced cell death via the apoptosis pathway. Cowanin treatment increased BCL2 associated X (Bax) and decreased Bcl-2 expression. In addition, cowanin activated caspase-9, -7 and poly-ADP-ribose-polymerase expression. Furthermore, cowanin decreased the levels of phosphorylated extracellular signal-regulated kinase (p-ERK), p-Akt, p-3-phosphoinositide-dependent protein kinase-1, while it increased p-p38 expression, thus resulting in the induction of apoptosis. In conclusion, cowanin inhibited cell proliferation and induced apoptosis of LoVo cells via the MAPK and Akt signaling pathways. Notably, inhibition of p38 by using a p38 inhibitor (SB203580) prevented the cowanin-induced apoptosis in LoVo cells. These results suggested that cowanin may be a potential candidate for the treatment of colorectal cancer and provided important information on the molecular mechanisms underlying its antitumor activity. © 2018 Spandidos Publications. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13177
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045395183&doi=10.3892%2fijo.2018.4353&partnerID=40&md5=79a225755790f03dd39f67ff640236ce
ISSN: 10196439
Appears in Collections:Scopus 1983-2021

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