Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13092
ชื่อเรื่อง: Development of monoclonal antibodies specific to ToxA and ToxB of Vibrio parahaemolyticus that cause acute hepatopancreatic necrosis disease (AHPND)
ผู้แต่ง: Wangman P.
Chaivisuthangkura P.
Sritunyalucksana K.
Taengchaiyaphum S.
Senapin S.
Pengsuk C.
Sithigorngul P.
Longyant S.
Keywords: antibody
bacterial disease
bacterium
epidemic
immunization
molecular analysis
rodent
symptom
toxin
China
Malaysia
Thailand
Viet Nam
Bacteria (microorganisms)
Decapoda (Crustacea)
Vibrio
Vibrio alginolyticus
Vibrio parahaemolyticus
วันที่เผยแพร่: 2017
บทคัดย่อ: Toxin A (ToxA) and toxin B (ToxB) of Vibrio parahaemolyticus, which cause acute hepatopancreatic necrosis disease (AHPND), were prepared in a bacterial supernatant from Chinese isolates (CN-VPAHPND) by washing bacterial colonies off of TSA cultures. The supernatant was subsequently used in mouse immunization to produce monoclonal antibodies (MAbs). Three groups of MAbs were selected: one MAb specific to ToxA, two MAbs specific to ToxB and one MAb specific to V. parahaemolyticus (CN-VPAHPND). The MAbs specific to ToxA and ToxB recognized all 10 VPAHPND isolates from China, Vietnam, Malaysia and Thailand, but did not bind to the 20 non-VPAHPND isolates from various other sources, including Vibrio spp. and other bacteria. The MAbs specific to toxins were used to detect the recombinant proteins of His-tagged ToxA and GST-ToxB with sensitivities of 200fmolspot−1 and 10fmolspot−1, respectively, as determined by dot-ELISA. The MAbs were used to detect the toxins produced by bacteria or shrimp tissue lysate spiked with bacteria in a complex tissue sample at concentrations as low as 1CFUml−1 after pre-enrichment of the bacteria for 6h. The third group of MAb was specific to CN-VPAHPND isolate but did not recognize the other 9 out of 10 VPAHPND isolates from Vietnam, Malaysia and Thailand. However, this MAb demonstrated cross-reactivity to 1 out of 20 of the non-VPAHPND isolates and 3 out of 9 of the V. alginolyticus isolates. These findings indicate that the AHPND epidemic in Southeast Asia was not caused by the CN-VPAHPND isolate. The MAbs specific to ToxA and ToxB produced in this study could be used to detect both toxins directly by dot blotting. © 2017 Elsevier B.V.
URI: https://ir.swu.ac.th/jspui/handle/123456789/13092
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85016138657&doi=10.1016%2fj.aquaculture.2017.03.039&partnerID=40&md5=c36e86c438114d0d9683bdf516440c8d
ISSN: 448486
Appears in Collections:Scopus 1983-2021

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