Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/13082
ชื่อเรื่อง: Characterization of PmSpӓtzle 1 from the black tiger shrimp Peneaus monodon
ผู้แต่ง: Boonrawd S.
Mani R.
Ponprateep S.
Supungul P.
Masrinoul P.
Tassanakajon A.
Rimphanitchayakit V.
Keywords: ALFPm3 protein
complementary DNA
crustinPm1 protein
crustinPm7 protein
DNA fragment
isoprotein
penaeidin3 protein
penaeidin5 protein
pmspatzle 1
protein
toll like receptor
unclassified drug
antimicrobial cationic peptide
arthropod protein
complementary DNA
isoprotein
amino acid sequence
animal cell
animal tissue
Article
blood cell
comparative study
controlled study
eye tissue
Fenneropenaeus chinensis
gene expression
heart tissue
hemolymph
hepatopancreas
ichthyophthiriasis
intestine tissue
lymphoid tissue
muscle tissue
nonhuman
nucleotide sequence
Penaeus monodon
protein analysis
signal transduction
stomach tissue
upregulation
Western blotting
White spot syndrome virus
animal
chemistry
gene expression
genetics
immunology
innate immunity
metabolism
molecular cloning
Penaeidae
physiology
sequence alignment
virology
Amino Acid Sequence
Animals
Antimicrobial Cationic Peptides
Arthropod Proteins
Base Sequence
Cloning, Molecular
DNA, Complementary
Gene Expression
Hemocytes
Immunity, Innate
Penaeidae
Protein Isoforms
Sequence Alignment
White spot syndrome virus 1
วันที่เผยแพร่: 2017
บทคัดย่อ: Spätzle is a signaling ligand in innate immune response that signals pathogenic infection via Toll receptor and Toll pathway into the cells for the synthesis of antimicrobial proteins. Herein, three PmSpӓtzle isoforms were identified in Penaeus monodon, namely PmSpz1, 2 and 3. The PmSpz1 was chosen for detailed study. The PmSpz1 gene was expressed in all nine tissues tested including the hemocytes, stomach, hepatopancreas, gill, lymphoid tissue, eyestalk, muscle, intestine and heart. Its expression was up-regulated upon white spot syndrome virus (WSSV) infection. Western blot analysis of hemolymph showed that the PmSpz1 mostly existed as a cleaved active form awaiting to activate the Toll pathway. Injection of a recombinant PmSpz1 rendered the shrimp less susceptible to the WSSV infection. Injection of a recombinant active form of PmSpz1 into a normal shrimp activated the synthesis of crustinPm1, crustinPm7, ALFPm3, penaeidin3 but not penaeidin5 indicating that the expression of all antimicrobial proteins but not penaeidin5 was under the regulation of Toll pathway. © 2017 Elsevier Ltd
URI: https://ir.swu.ac.th/jspui/handle/123456789/13082
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85017449346&doi=10.1016%2fj.fsi.2017.04.005&partnerID=40&md5=6e8a109052eabdc84ea47ec277170f54
ISSN: 10504648
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.