Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12970
ชื่อเรื่อง: Optimization of Poly(dl-Lactic Acid) Degradation and Evaluation of Biological Re-polymerization
ผู้แต่ง: Youngpreda A.
Panyachanakul T.
Kitpreechavanich V.
Sirisansaneeyakul S.
Suksamrarn S.
Tokuyama S.
Krajangsang S.
Keywords: Biodegradation
Enzyme activity
Enzymes
Global warming
Lactic acid
Polymerization
Waste utilization
Biodegradable plastics
Biological degradation
Biological recycling
Central composite designs
Global warming problems
Optimized conditions
Poly(dl-lactic acid)
Protease
Biodegradable polymers
biodegradation
catalyst
enzyme activity
optimization
plastic waste
polymer
polymerization
Actinomadura
วันที่เผยแพร่: 2017
บทคัดย่อ: Poly(dl-lactic acid) or PLA is a biodegradable polymer. It has received much attention since it plays an important role in resolving the global warming problem. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported as having PLA depolymerase potential and being applicable to PLA biodegradation, which was used in this work. Therefore, this research demonstrates the important basic knowledge on the biological degradation process by the crude PLA-degrading enzyme from strain T16-1. Its re-polymerization was evaluated. The optimization of PLA degradation by statistical methods based on central composite design was determined. Approximately 6700 mg/l PLA powder was degraded by the crude enzyme under optimized conditions: an initial enzyme activity of 200 U/ml, incubated at 60 °C for 24 h released 6843 mg/l lactic acid with 82% conversion, which was similar to the commercial enzyme proteinase K (81%). The degradable products were re-polymerized repeatedly by using commercial lipase as a catalyst under a nitrogen atmosphere for 6 h. A PLA oligomer was achieved with a molecular weight of 378 Da (n = 5). This is the first report to demonstrate the high efficiency of the enzyme to degrade 100% of PLA powder and to show the biological recycling process of PLA, which is promising for the treatment and utilization of biodegradable plastic wastes in the future. © 2016, Springer Science+Business Media New York.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12970
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84995475642&doi=10.1007%2fs10924-016-0885-1&partnerID=40&md5=3871c7bd72cf66b2df029b9e6c5ecf91
ISSN: 15662543
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.