Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12954
ชื่อเรื่อง: Doxorubicin-loaded micelle targeting MUC1: A potential therapeutic for MUC1 triple negative breast cancer treatment
ผู้แต่ง: Khondee S.
Chittasupho C.
Tima S.
Anuchapreeda S.
Keywords: doxorubicin
mucin 1
antineoplastic antibiotic
doxorubicin
drug carrier
MUC1 protein, human
mucin 1
peptide
Article
breast cancer cell line
cell viability
cytotoxicity
drug binding
drug delivery system
drug efficacy
drug mechanism
high performance liquid chromatography
human
human cell
micelle
particle size
peptide synthesis
photon correlation spectroscopy
priority journal
protein targeting
transmission electron microscopy
triple negative breast cancer
cell survival
drug effect
drug release
metabolism
micelle
tumor cell line
Antibiotics, Antineoplastic
Cell Line, Tumor
Cell Survival
Doxorubicin
Drug Carriers
Drug Liberation
Humans
Micelles
Mucin-1
Peptides
Triple Negative Breast Neoplasms
วันที่เผยแพร่: 2018
บทคัดย่อ: Background: Triple negative breast cancer (TNBC) is an aggressive disease associated with poor prognosis and lack of validated targeted therapy. Thus chemotherapy is a main adjuvant treatment for TNBC patients, but it associates with severe toxicities. For a better treatment outcome, we developed an alternative therapeutic, doxorubicin (DOX)-loaded micelles targeting human mucin1 protein (MUC1) that is less toxic, more effective and targeted to TNBC. Methods: From many candidate peptides, QNDRHPR-GGGSK (QND) and HSQLPQV-GGGSK (HSQ) were identified computationally, synthesized and purified using solid phase peptide synthesis and semipreparative HPLC. The peptides showed significant high binding to MUC1 expressing cells using a fluorescent microscope. The peptides were then conjugated on pegylated octadecyl lithocholate copolymer. DOX-encapsulated micelles were formed through self-assembly. MUC1-targeted micelles were characterized using dynamic light scattering (DLS) and Transmission Electron Microscopy (TEM). Drug entrapment efficiency was examined using a microplate reader. Cytotoxicity, binding, and uptake were also investigated. Results: Two types of DOX-loaded micelles with different targeting peptides, QND or HSQ, were developed. DOX-loaded micelles were spherical in shape with average particle size around 300-320 nm. Drug entrapment efficiency of untargeted and targeted DOX micelles was about 71-93%. Targeted QND-DOX and HSQ-DOX micelles exhibited significantly higher cytotoxicity compared to free DOX and untargeted DOX micelles on BT549-Luc cells. In addition, significantly greater binding and uptake were observed for QND-DOX and HSQ-DOX micelles on BT549-Luc and T47D cells. Conclusion: Taken together, these results suggested that QND-DOX and HSQ-DOX micelles have a potential application in the treatment of TNBC-expressing MUC1. © 2018 Bentham Science Publishers.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12954
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85045011068&doi=10.2174%2f1567201814666170712122508&partnerID=40&md5=3c2c42febfba0fb4d2be61cf0cebe3be
ISSN: 15672018
Appears in Collections:Scopus 1983-2021

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