Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12889
ชื่อเรื่อง: Molecular isolation and characterization of a haemocyanin of Macrobrachium rosenbergii reveal its antibacterial activities
ผู้แต่ง: Srisuk C.
Senapin S.
Bendena W.G.
Longyant S.
Sithigorngul P.
Chaivisuthangkura P.
Keywords: Aeromonas punctata
Bacillus cereus
Bacteria (microorganisms)
Crustacea
Decapoda (Crustacea)
Exopalaemon carinicauda
Macrobrachium nipponense
Macrobrachium rosenbergii
Macrobrachium rosenbergii nodavirus
Vibrio vulnificus
วันที่เผยแพร่: 2018
บทคัดย่อ: Haemocyanin is a multi-subunit protein complex found in the haemolymph and is involved in the immune system of crustaceans. In this study, a haemocyanin gene of Macrobrachium rosenbergii, designated MrHc, was successfully isolated. The MrHc gene contained an open reading frame (ORF) of 1,992 nucleotides, encoding a protein of 663 amino acid residues with a molecular mass of 76.5 kDa. The deduced amino acid sequence contained distinct structural motifs of the haemocyanin superfamily, including an all-alpha domain, a copper-containing domain and an immunoglobulin-like domain. Based on the phylogenetic analysis, the MrHC protein demonstrated a close relationship with the haemocyanins of Palaemon carinicauda and Macrobrachium nipponense. The MrHc gene was expressed in various shrimp tissues, including the hepatopancreas, gill, haemocytes, stomach and muscle. After Macrobrachium rosenbergii nodavirus (MrNV) challenge tests, the MrHc gene was up-regulated 237-fold at day 2. A recombinant protein of the MrHc immunoglobulin-like domain exhibited antibacterial activity against Vibrio vulnificus, V. parahaemolyticus, Aeromonas caviae, A. veronii, A. hydrophila and Bacillus cereus. This study suggested that MrHc may play important roles in the shrimp innate immune response to MrNV infection and bacterial infection. © 2017 John Wiley & Sons Ltd
URI: https://ir.swu.ac.th/jspui/handle/123456789/12889
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029396157&doi=10.1111%2fare.13481&partnerID=40&md5=09e55e4696efa922710a610337bd1423
ISSN: 1355557X
Appears in Collections:Scopus 1983-2021

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