Please use this identifier to cite or link to this item: https://ir.swu.ac.th/jspui/handle/123456789/12781
ชื่อเรื่อง: Optimization, purification and characterization of β-xylanase by a novel thermotolerant strain of microbispora siamensis, DMKUA 245T
ผู้แต่ง: Boondaeng A.
Krajangsang S.
Trakunjae C.
Lomthong T.
Tokuyama S.
Kitpreechavanich V.
วันที่เผยแพร่: 2018
บทคัดย่อ: Investigation of factors affecting β-xylanase production by a newly thermotolerant strain of Microbispora siamensis, DMKUA 245T, using a sequence of statistical methods, indicated that the optimized medium was composed of 10 g/L of xylan, 0.7 g/L of casein, 1 g/L of K2HPO4 and 0.15 g/L of MgSO4⋅7H2O, yielding 44 U/mL of β-xylanase activity in a shaking flask at a temperature of 35 °C for 120 h. This was a 4.4-fold increase compared to when using unoptimized medium. β-Xylanase production of 250 U/mL was obtained in a 3 L stirrer fermenter with an aeration rate of 0.5 vvm, agitation speed of 150 rpm and initial pH of 7.0 for 72 h cultivation. The β-xylanase enzyme from the strain DMKUA 245T was purified, with a specific activity of 219.4 U/mg protein obtained. SDS-PAGE and gel filtration indicated that the molecular weight of the purified β-xylanase as a monomer was estimated to be 65.8 kDa. The optimum pH and temperature for β-xylanase activity were 5.5 and 60 °C, respectively. The activity of Co2+-, K+-and Mg2+-treated purified enzymes was more thermostable than the untreated purified enzyme, particularly the Co2+-treated purified enzyme, which was still stable at temperatures up to 90 °C. The hydrolysis products were a series of short-chain xylooligosaccharides, indicating that the purified enzyme was an endo-β-1, 4-xylanase. © 2018, Chiang Mai University. All rights reserved.
URI: https://ir.swu.ac.th/jspui/handle/123456789/12781
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056430126&partnerID=40&md5=822b9b401363b9914644f13e6b7c16cc
ISSN: 1252526
Appears in Collections:Scopus 1983-2021

Files in This Item:
There are no files associated with this item.


Items in SWU repository are protected by copyright, with all rights reserved, unless otherwise indicated.